Screening Lipolytic from Soil Bacterial Contaminated Oil

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Maylina Ilhami Khurniyati Sri Sumarsih Uswatun Chasanah

Abstract

Lipase (triasilglycerol acylhydrolase, E.C, 3.1.1.3) is an enzyme can hydrolyze long chains of triglycerides[1]. Lipase is a water-soluble enzyme and naturally lipase catalyze the hydrolysis of ester bonds in water insoluble lipid substrate and long-chain acylglycerol synthesis, diacylglycerol, monoacylglycerol, and glycerol[2]. Hydrolase enzyme lipase can be used potentialy produced fatty acid which precursors in the chemical industry[3]. The research aims to study the lipolytic activity of bacteria isolated from soil contaminated with oil. Screening of lipolytic bacteria was performed in Rhodamin-B agar plate containing olive oil. The lipolytic activity was determined by spectrofotometry method toward p-nitrofenil palmitat as a substrate. The result this research showed that there are 15 of 150 isolates bacteria from soil contaminated with oil was confirmed by observing the zone of hydrolysis formed around the growth of colony on Rhodamin B agar plate containing olive oil. One of the lipolytic bacteria identified as higher activity  was produced lipase with activity of 7,41 U/ml
 

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How to Cite
Khurniyati, M., Sumarsih, S., & Chasanah, U. (2022). Screening Lipolytic from Soil Bacterial Contaminated Oil. ALKIMIA : Jurnal Ilmu Kimia Dan Terapan, 6(1), 224-228. https://doi.org/https://doi.org/10.19109/alkimia.v6i1.12297
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References

[1] Shariff, Farolniza Mohd., Raja Noor Zaliha Raja Abd Rahman., Mahiran, Basri., and Abu, Bakar Salleh. 2011. A newly Isolated Thermostable Lipase from Bacillus sp. Malaysia.
[2] Miller,J., Leslie D. Patterson. 2010. Enzymatic Deprotection of the Cephalosporin 3′-Acetoxy Group Using Candida antarctica Lipase B. The Journal Of Organic Chemsitry. 75(4):1289-1292. https://doi.org/10.1021/jo902406b
[3] Gilham D., Lehner R. 2005. Techniques to measure lipase and esterase activity in vitro.Methods 36:139-147. doi:10.1016/j.ymeth.2004.11.003.
[4] Zuridah, H, H. Norazwin, M.N. Aisyah, M.N.A. Fakhruzzaman, and N.A. Zeenathul. 2011. Identification of lipase producing thermophilic bacteria from Malaysian hot springs . Acad. J. Microbiol. Res., 5 (21): 3569-3573. DOI:10.5897/AJMR11.777
[5] Seftiono, 2017. Determination of Mananase Enzyme Activities from Various Microorganisms in Indonesia and Its Role in the Food Sector: Literature Review. Agrointek 11 (1) : 14-20. doi : 10.21107/agrointek.v11i1.2939
[6] Wirawan, 2018. Lipase Activity of Endophytic Actinobacteria from Medicinal Plants. HAYATI Journal of Biosciences 25(1):1-5. doi : https://doi.org/10.4308/hjb.25.1.1
[7] Zulfahair, T. Setyaningtyas., dan A. Fatoni. 2010. solation, Purification, and Characterization of Screened Bacterial Lipases from the Final Disposal Soil (TPA) of Gunung Tugel Banyumas. Jurnal Nature Indonesia,12(2) : 124-129. doi : http://dx.doi.org/10.31258/jnat.12.2.124-129
[8] Gupta, V.K., Kumar, D., Kumar, L.,Nagar, S., Raina, C., and Parshad, R. 2012. Screening, Isolation, and Production Lipase Producing Bacillus sp. Strain DVL2 and its Potential Evaluation in Esterification and Resolution Actions. Achieves of Apllied Science Research, 4(4):1736-1770.