Antibacterial Activity Test of Wet and Dried Extracts of Calabash Tree (Crescentia cujete L.) against Aeromonas hydrophilla Natural drug use extracts of calabash tree to control the growth of Aeromonas hydrophilla by In vitro

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Noorkomala Sari Nengah Dwianita Kuswytasari Awik Puji Dyah Nurhayati

Abstract

Study to determine the antibacterial activity of wet and dry extract of the leaf, fruit, and bark of Calabash tree (Crescentia cujete L.) against the growth of Aeromonas hydrophila. The solvent extraction process was done by using 96% ethanol in the maceration method. Antibacterial test results using diffusion agar to decide clear zone and tube series of dilution test to provide MIC and MBC. Fresh leaf extract produces the highest clear zone diameter (20.06 mm), after which fresh bark extract (12.81 mm), and the last is fresh fruit extract (3.22 mm). In contrast to fresh extracts, the dried extracts are have not clear zone. MIC (Minimum Inhibitory Concentration) of Calabash Tree fresh leaf extract against Aeromonas hydrophila is 80%, and MBC (Minimum Bactericidal Concentration) is 100%.

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Sari, N., Kuswytasari, N., & Nurhayati, A. (2020). Antibacterial Activity Test of Wet and Dried Extracts of Calabash Tree (Crescentia cujete L.) against Aeromonas hydrophilla. Jurnal Biota, 6(1), 5-11. Retrieved from http://jurnal.radenfatah.ac.id/index.php/biota/article/view/3954
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References

Gillespie, S. H. (1994). Medical Microbiology Illustrated. London: Butterworth Heinemann.
Aulia, I. (2008). Uji Aktifitas Antibakteri Fraksi Etil Asetat Ekstark Etanolik Daun Arbenan (Duschesnea indica (Andr.) Focke) Terhadap Staphylococcus aureus dan Pseudomonas aeruginosa Multiresisten Antibiotik Beserta Profil Kromatografi Lapis Tipisnya. Universitas Muhammadiyah Surakarta, Fakultas Farmasi. Surakarta: Universitas Muhammadiyah Surakarta.
Anaf. (2009, Nopember 20). Retrieved Nopember 20, 2009, from Majapahit (Crescentia cujeteL.): http://anaf.blogspot.com/
Boyd, R. F. (1995). Basic Medical Microbiology (Fifth ed.). USA: Little Brown Company.
Cushine, T. T., & Lamb, A. J. (2005). Antimicrobial Activity of Flavonoids. International Journal of Antimicrobial Agents , 26, 343-356.
Cushnie, T., Cushnie, B., & Lamb, A. (2014). Alkaloids: An Overview of their antibacterial, antibiotic-enhancing and antivirulance activities. International Journal of Antimicrobial Agents , 44 (5), 377-386.
Greenwood. (1995). Antibiotics Susceptibility Illustrated Test, Antimicrobial and Chemoteraphy. USA: Mc Graw Hill Company.
Intan, S. M. (2008). the Activity of Leaf Extract Calabash Tree (Cescentia cujete L.) againts Staphylococcus aureus and Streptococcus pyogenes by in vitro. Sepuluh Nopember Institute of Technology, Department of Biology. Surabaya: Sepuluh Nopember Institute of Technology.
Hutapea, J. R. (1993). Inventaris Tanaman Obat Indonesia III. Jakarta: Badan Penelitian dan Pengembangan Kesehatan.
Jewetz, M., & Adelberg's. (1996). Medical Microbiology (20 ed.). Jakarta: Buku Kedokteran EGC.
Kusumawardani. (2007). Daya Anti Bakteri Ekstrak Jahe Merah (Zingiher officinale Rosc.) dengan Konsentrasi yang Berbeda terhadap Pertumbuhan Aeromonas hyrdrophilla Secara In Vitro. Surabaya, Jawa Timur, Indonesia: Fakultas Kedokteran Hewan Universitas Airlangga.
Kordi, & Ghufran. (2004). Penanggulangan Hama dan Penyakit Ikan. Jakarta, Indonesia: PT BIna Adiaksara.
Krishnan, N., Ramanathan, S., Sasidharan, S., Murugaiyah, V., & Mansor, S. M. (2010). Antimicrobial Activity Evaluation of Cassia spectabilis Leaf. International Journal of Pharmacology , 6 (4), 510-514.
Lien, F. N. (2001). Uji Toksisitas Ekstrak Metanol Daun Beremuk (Crescentia cujeteL.) terhadap Larva Artemia
salina Leach. Universitas Surabaya, Fakultas Farmasi. Surabaya: Fakultas Farmasi Universitas Surabaya.
Nielsen, Slamet, & Wege. (2009). The Synthesis of 3-hydroxymethylfurol [3,2-b] naptho [2,3-d] furan-5,10dione, A Novel Metabolite isolated from Crescentia cujete. Journal of Tetrahedron , 65, 4569-4577.
Noer, I. S., & Nurhayati, L. (2006). Bioaktivitas Ulva reticulate Forsskal Asal Gili Kondo Lombok Timur terhadap Bakteri. Jurnal Biotika , 6 (1), 45-60.
Oghubuagu, M. N. (2008). The Nutritive and Anti-nutritive Compositions of Calabash (Crescentia cujete ) Fruit Pulp. Journal of Animal and Veterinary Advances , 7 (9), 45-60.
Osata, M. S., Reddy, G. S., Penland, L. R., & Graham, Y. D. (2001). Comaprison of The Etes and the NCCLS-approved agar dilution method to detect metronidazole and clarithromycin resistant Heliobacter pylori. International Journal of Antimicrobial Agents , 17, 39-44.
Purwaninigsih, & Suwidah. (2007). Kerusakan Jaringan pada Ikan Kancra (Tor sp.) Akibat Infeksi Artificial Bakteri Aeromonas hyrophila. Seminar Nasional Perikanan UGM. Yogyakarta: UGM Press.
Pachanawan, A., Parichat, P., & Pongsok, R. (2008). Potential of Psidium guajava Supplemented Fish Diets in Controllin Aeromonas hydrophilla Infection in Tilapia (Oreachromis niloticus). Journal of Bioscience and Bioengineering , 106 (5), 419-424.
Pelczar, M. J., & Chan, E. (2005). Dasardasar Mikrobiologi. Jakarta: Universitas Indonesia Press.
Poeloengan, M., Chairul, Iyep, K., Siti, S., & Susan, M. N. (2006). Aktivitas Antimikroba dan Fitokimia dari Beberapa Tanaman Obat. Seminar Nasional Teknologi Peternakan dan Veteriner. Institut Pertanian Bogor.
Scorzoni, L., Benaducci, T., Almeida, A. F., Silva, D. S., Bolzani, V., & Gianinni, M. S. (2007). The Use of Standard Methodology for Determination of Antifungal Activity of Natural Products Againts Medical Yeast Candida Sp. and Cryptococcus Sp. Brazilian Journal of Microbiology , 38, 391-397.
Wattimena, J. R., Sugiarso, N. C., Widianto, M. B., Sukandar, E. Y., Soemardji, A. A., & Setiabudi, A. R. (1991). Farmakologi dan Terapi Antibiotik. Yogyakarta: Gadjah Mada University Press.