Endophyte Extract From Nattle (Urtica dioica L.) Against Staphylococcus aureus and Escherichia coli
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Published:
Sep 12, 2022
Keywords:
Endophytes, Nettle stem, Antibacterial
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Abstract
Endophytes are microorganisms that colonize the plant host tissue. Endophytic colonies are able to produce secondary metabolites in accordance with their host plants, endophytes have been found in every plant species studied to date and are recognized as a source of new natural compounds that have the potential to be exploited in medicine. This study aims to isolate and obtain endophytic fungi and to test secondary metabolites that have the potential as antibacterial against Staphylococcus aureus and Escherichia coli. Plant samples were taken around the village of Mamben, Wanasaba. The research method used is experimental laboratory. This research was conducted by isolating and identifying endophytic fungi from nettle stems. Obtained a type of endophytic fungal strain using Potato Dextrose Agar (PDA) media. The production of secondary metabolites of endophytic fungi was obtained by fermentation method using brown rice for a month then macerated for 3 days using methanol solvent and tested for secondary metabolites and antibacterial activity. The endophytic fungal extract of nettle stem contains alkaloids and flavonoids and is able to inhibit the growth of Staphylococcus aureus bacteria with the highest concentration of 60% with an inhibition zone of 18 mm. whereas, the extract of nettle stem endophytic fungus on Escherichia coli has not an inhibition zone.
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Endophyte Extract From Nattle (Urtica dioica L.) Against Staphylococcus aureus and Escherichia coli. (2022). ALKIMIA : Jurnal Ilmu Kimia Dan Terapan, 6(1), 196-203. https://doi.org/10.19109/alkimia.v6i1.11277
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How to Cite
Endophyte Extract From Nattle (Urtica dioica L.) Against Staphylococcus aureus and Escherichia coli. (2022). ALKIMIA : Jurnal Ilmu Kimia Dan Terapan, 6(1), 196-203. https://doi.org/10.19109/alkimia.v6i1.11277
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[4] E. Nurnasari, K. Sri Wijayanti, and A. Riset, “Aktivitas Antibakteri Minyak Atsiri Daun Tembakau terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus Antibacterial Activities of Tobacco Leaf Essential Oil Against Escherichia coli and Stapphylococcus aureus,” J. Kefarmasian Indones., vol. 9, no. 1, pp. 48–56, 2019.
[5] R. X. Tan and W. X. Zou, “Tan&Zou(2001).pdf,” endophytes a rich source fuctional Metab. Nat Pro.Rep., no. March, pp. 448–459, 2001.
[6] strobel G and Daisy B, “Bioprospecting for microbial endophytes and yheir natural products,” Microbiol. Mol. Biol., vol. 67, pp. 491–502, 2003.
[7] H. Kregiel, D., Pawlikowska, E., & Antolak, “Urtica spp.: Ordinary plants with extraordinary properties,” Mol. A J. Synth. Chem. Nat. Prod. Chem., vol. 23, no. 1664, 2018.
[8] N. N. Fadilah and S. Susanti, “Aktivitas Antihiperurisemia Ekstrak Tanaman Jelatang (Urtica dioca L.) pada Mencit,” Heal. Inf. J. Penelit., vol. 12, no. 1, pp. 99–106, 2020.
[9] A. Ketema and A. Worku, “Antibacterial Finishing of Cotton Fabric Using Stinging Nettle (Urtica dioica L.) Plant Leaf Extract,” J. Chem., vol. 2020, 2020.
[10] & A. Azim, Shiono, “Eksplorasi Jamur Endofit Dari Tanaman Kerinyu (Cromolaena odorata L.) Dampak Stres Lingkungan Serta Aktifitas Anti Bakteri Dan Anti Jamurnya,” Kim. Pendidik. Kim., vol. 3, no. 85, pp. 1–11, 2021, doi: 10.20414/spin.v3i1.3108.
[11] Ergina, S. Nuryanti, and I. D. Purtisari, “Uji Kualitatif Senyawa Metabolit Sekunder Pada Daun Palado (Agave angustifolia) Yang Diekstraksi Dengan Pelarut Air Dan Etanol Qualitative Test of Secondary Metabolites Compounds in Palado Leaves (Agave),” J. Akad. Kim, vol. 3, no. 3, pp. 165–172, 2014.
[12] E. S. F. Noverita, Dinah Fitria, “Isolasi Dan Uji Aktivitas Antibakteri Jamur Endofit Dari Daun Dan Rimpang,” Farm. Indones., vol. 4, no. April, pp. 171–176, 2009.
[13] R. R. S. Susanto, Sudrajat, “kandungan bahan aktif tumbuhan meranti merah (Shorea leprosula Miq) sebagai sumber senyawa antibakteri,” Mulawarman Sci, vol. 11, no. (12), pp. 181–90, 2012.
[14] Y. A. Kasi, J. Posangi, O. M. Wowor, and R. Bara, “Uji Efek Antibakteri Jamur Endofit Daun Mangrove Avicennia Marina Terhadap Bakteri Uji Staphylococcus Aureus Dan Shigella Dysenteriae,” J. e-Biomedik, vol. 3, no. 1, 2015.
[15] J. Elviasari and R. Rusli, “Identifikasi Metabolit Sekunder Dan Uji Aktivitas Antibakteri Isolat Jamur Endofit Daun Beluntas (Pluchea indica (L.) Less.),” J. Sains dan Kesehat., vol. 1, no. 5, pp. 214–220, 2015.
[16] S. D. Kumar A. Singh R., Yadav A., “Isolation and characterization of bacterial endophytes of Curcuma longa L,” 3 Biotech 6, vol. 1–8, 2016.
[17] H. L. Barnet, Illustrated Genera of Imperfect Fungi, Second Edi. Burgess Publishing Company, 1972.