Primers Designed For Amplifying TCF7L2 Gen
Article Sidebar
Published
Aug 31, 2020
Main Article Content
Abstract
Diabetes Mellitus (DM) is a disease characterized by high glucose levels because of the lack of insulin hormone in the body. Prevalence of Type-2 Diabetes Mellitus is associated with the variant of the TCF7L2 gene polymorphism, or SNP. The purpose of this study was to construct a specific primer to amplify the TCF7L2 gene fragment and their capabilities to detect the targeted area. This research was a descriptive study that design the primers using Geneious software version 5.4.7. The study was conducted at the Laboratory of Genetics and Biotechnology FMIPA Universitas Negeri Padang. Based on this research, a 10qF forward primer (NG_012631.1) and 10qR reverse primers (NG_012631.1) has successfully designed. The primers can amplify the targeted area of the TCF7L2 gene with 1208 bp length. The amplified gene region has many variants that are strictly related to the appearance of Type 2 Diabetes Mellitus.
Article Details
How to Cite
Syamsurizal, S., Saputra, H., Putri, D., & Badriyya, E. (2020). Primers Designed For Amplifying TCF7L2 Gen. Jurnal Biota, 6(2), 63-70. https://doi.org/https://doi.org/10.19109/10.19109/Biota.v6i2.5871
Section
Artikel
The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to journal Biota Faculty Sains and Tecnology, Universitas Islam Negeri Raden Fatah Palembang as publisher of the journal, and the author also holds the copyright without restriction.
Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , are allowed with a written permission from journal Biota Faculty Sains and Tecnology, Universitas Islam Negeri Raden Fatah Palembang.
Jurnal BiotaBoard, Faculty of Sains and Tecnology, the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal. In any way, the contents of the articles and advertisements published in the journal Biota Faculty of of Sains and Tecnology are sole and exclusive responsibility of their respective authors and advertisers.
Jurnal Biota by http://jurnal.radenfatah.ac.id/index.php/biota is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
Abuhendi, N., Qush, A., Naji, F., Abunada, H., Al Buainain, R., Shi, Z., & Zayed, H. (2019). Genetic polymorphisms associated with type 2 diabetes in the Arab world: A systematic review and meta-analysis. Diabetes Research and Clinical Practice, 151, 198–208. https://doi.org/10.1016/j.diabres.2019.03.037
American Diabetes Association. (2017). Standards of Medical Care in Diabetes–2017.
Borah, P. (2011). Primer designing for PCR. Science Vision, 11(3), 134–136.
Cook, J. P., & Morris, A. P. (2016). Multi-ethnic genome-wide association study identifies novel locus for type 2 diabetes susceptibility. European Journal of Human Genetics, 24(8), 1175–1180. https://doi.org/10.1038/ejhg.2016.17
Departemen Kesehatan Republik Indonesia. (2013). Laporan Riset Kesehatan Dasar (Riskesdas), Bagian Penyakit Tidak Menular. jakarta: Badan Litbangkes, Depkes RI.
Diabetes Federation International. (2019). IDF Diabetes Atlas 2019. In International Diabetes Federation. Retrieved from http://www.idf.org/about-diabetes/facts-figures
Elrod, S. L., & Stansfield, W. D. (2007). Genetika (4th editio). jakarta: erlangga.
Erkoç Kaya, D., Arikoğlu, H., Kayiş, S. A., Öztürk, O., & Gönen, M. S. (2017). Transcription factor 7-like 2 (TCF7L2) gene polymorphisms are strong predictors of type 2 diabetes among nonobese diabetics in the Turkish population. Turkish Journal of Medical Sciences, 47(1), 22–28. https://doi.org/10.3906/sag-1507-160
Fahmiyah, I., & Latra, N. I. (2016). Faktor yang Mempengaruhi Kadar Gula Darah Puasa Pasien Diabetes Mellitus Tipe 2 di Poli Diabetes RSUD Dr. Soetomo Surabaya Menggunakan Regresi Probit Biner. Jurnal Sains Dan Seni ITS, 5(2), 2337–3520.
Franca, L. T. C., Carrilho, E., & Kist, T. B. L. (2002). A review of DNA sequencing techniques. Quarterly Review of Biophysics, 35(2), 169–200.
Ghassibe-Sabbagh, M., Haber, M., Salloum, A. K., Al-Sarraj, Y., Akle, Y., Hirbli, K., … Zalloua, P. A. (2014). T2DM GWAS in the Lebanese population confirms the role of TCF7L2 and CDKAL1 in disease susceptibility. Scientific Reports, 4, 7351. https://doi.org/10.1038/srep07351
Grant, S. F. A., Thorleifsson, G., Reynisdottir, I., Benediktsson, R., Manolescu, A., Sainz, J., … Stefansson, K. (2006). Variant of transcription factor 7-like 2 (TCF7L2) gene confers risk of type 2 diabetes. Nature Genetics, 38(3), 320–323. https://doi.org/10.1038/ng1732
Huzaimi, M. (2009). Association of TCF7L2 variants with type 2 diabetes mellitus susceptibility and treatment outcome in a multi-ethnic malaysian population. University of Malaya, Kuala Lumpur.
Jin, T., & Liu, L. (2008). The Wnt signaling pathway effector TCF7L2 and type 2 diabetes mellitus. Molecular Endocrinology, 22(11), 2383–2392. https://doi.org/10.1210/me.2008-0135
Kariadi, & Sri Hartini KS. (2009). Diabetes? Siapa Takut!! Panduan Lengkap Untuk Diabetisi, Keluarganya, dan Profesional Medis. Bandung: PT. Mizan Pustaka.
Radha, V., & Mohan, V. (2007). Genetic predisposition to type 2 diabetes among Asian Indians. The Indian Journal of Medical Research, 125(3), 259–274.
Sale, M., Smith, S. G., Mychaleckyj, J. C., Keene, K. L., Langefeld, C. D., Leak, T. S., … Freedman, B. I. (2007). Gene Are Associated With Type 2 Diabetes in an African-American Population Enriched for Nephropathy. October, 56(October), 2638–2642. https://doi.org/10.2337/db07-0012.Additional
Soelistijo, S., Novida, H., Rudijanto, A., Soewondo, P., Suastika, K., Manaf, A., … Soetedjo, N. (2015). Konsesus Pengelolaan Dan Pencegahan Diabetes Melitus Tipe2 Di Indonesia 2015. In Perkeni. Retrieved from https://www.google.com/url?sa=t&source=web&rct=j&url=https://pbperkeni.or.id/wp-content/uploads/2019/01/4.-Konsensus-Pengelolaan-dan-Pencegahan-Diabetes-melitus-tipe-2-di-Indonesia-PERKENI-2015.pdf&ved=2ahUKEwjy8KOs8cfoAhXCb30KHQb1Ck0QFjADegQIBhAB&usg=AOv
Syamsurizal, & Kadri, H. (2018). Genotyping SNP Rs12255372 TCF7L2 Gene Using Three-Primer ARMS-PCR for Detection T2DM n Indonesian Batak Ethnic. Journal of Physics: Conference Series, 1040(1). https://doi.org/10.1088/1742-6596/1040/1/012003
Syamsurizal, S., Handayani, D., Kadri, H., & Badriyya, E. (2019). Genotyping SNP rs7903146 TCF7L2 gene for detection T2DM in Indonesian melayu ethnic Genotyping SNP rs7903146 TCF7L2 gene for detection T2DM in Indonesian melayu ethnic. Journal of Physics: Conference Series, 1317, 1–8. https://doi.org/10.1088/1742-6596/1317/1/012090
Syamsurizal, S., Yanwirasti, Manaf, A., & Jamsari. (2014). Konstruksi Primer untuk Deteksi SNP rs12255372 pada Gen Transcription Factor 7 Like 2 (TCF7L2) Penyebab Diabetes Melitus Tipe-2 dengan Metode Amplification Refractory Mutation System (ARMS)– PCR. Prosiding Seminar Nasional Dan Wordshop Perkembangan Terkini Sains Farmasi Klinik 3. Padang.
Tama, B. A., F.S., R., & Hermansyah. (2011). An early detection method of type-2 diabetes mellitus in public hospital. TELKOMNIKA, 9(2), 287–294.
Votsi, C., Toufexis, C., Michailidou, K., Antoniades, A., Skordis, N., Karaolis, M., … Christodoulou, K. (2017). Type 2 Diabetes Susceptibility in the Greek-Cypriot Population: Replication of Associations with TCF7L2, FTO, HHEX, SLC30A8 and IGF2BP2 Polymorphisms. Genes, 8(1). https://doi.org/10.3390/genes8010016
World Health Organization. (2016). Global Report on Diabetes. France.
Yao, D. D., Yang, L., Wang, Y., Liu, C., Wei, Y. J., Jia, X. B., … Shu, L. (2015). Geniposide promotes beta-cell regeneration and survival through regulating β-catenin/TCF7L2 pathway. Cell Death and Disease, 6(5), e1746-13. https://doi.org/10.1038/cddis.2015.107
Yusuf, Z. K. (2010). Polymerase Chain Reaction (PCR). Saintek, 5(6), 1–6.
Yuwono, T. (2006). Teori dan Aplikasi Polymerase Chain Reaktion. Yogyakarta: andi.
American Diabetes Association. (2017). Standards of Medical Care in Diabetes–2017.
Borah, P. (2011). Primer designing for PCR. Science Vision, 11(3), 134–136.
Cook, J. P., & Morris, A. P. (2016). Multi-ethnic genome-wide association study identifies novel locus for type 2 diabetes susceptibility. European Journal of Human Genetics, 24(8), 1175–1180. https://doi.org/10.1038/ejhg.2016.17
Departemen Kesehatan Republik Indonesia. (2013). Laporan Riset Kesehatan Dasar (Riskesdas), Bagian Penyakit Tidak Menular. jakarta: Badan Litbangkes, Depkes RI.
Diabetes Federation International. (2019). IDF Diabetes Atlas 2019. In International Diabetes Federation. Retrieved from http://www.idf.org/about-diabetes/facts-figures
Elrod, S. L., & Stansfield, W. D. (2007). Genetika (4th editio). jakarta: erlangga.
Erkoç Kaya, D., Arikoğlu, H., Kayiş, S. A., Öztürk, O., & Gönen, M. S. (2017). Transcription factor 7-like 2 (TCF7L2) gene polymorphisms are strong predictors of type 2 diabetes among nonobese diabetics in the Turkish population. Turkish Journal of Medical Sciences, 47(1), 22–28. https://doi.org/10.3906/sag-1507-160
Fahmiyah, I., & Latra, N. I. (2016). Faktor yang Mempengaruhi Kadar Gula Darah Puasa Pasien Diabetes Mellitus Tipe 2 di Poli Diabetes RSUD Dr. Soetomo Surabaya Menggunakan Regresi Probit Biner. Jurnal Sains Dan Seni ITS, 5(2), 2337–3520.
Franca, L. T. C., Carrilho, E., & Kist, T. B. L. (2002). A review of DNA sequencing techniques. Quarterly Review of Biophysics, 35(2), 169–200.
Ghassibe-Sabbagh, M., Haber, M., Salloum, A. K., Al-Sarraj, Y., Akle, Y., Hirbli, K., … Zalloua, P. A. (2014). T2DM GWAS in the Lebanese population confirms the role of TCF7L2 and CDKAL1 in disease susceptibility. Scientific Reports, 4, 7351. https://doi.org/10.1038/srep07351
Grant, S. F. A., Thorleifsson, G., Reynisdottir, I., Benediktsson, R., Manolescu, A., Sainz, J., … Stefansson, K. (2006). Variant of transcription factor 7-like 2 (TCF7L2) gene confers risk of type 2 diabetes. Nature Genetics, 38(3), 320–323. https://doi.org/10.1038/ng1732
Huzaimi, M. (2009). Association of TCF7L2 variants with type 2 diabetes mellitus susceptibility and treatment outcome in a multi-ethnic malaysian population. University of Malaya, Kuala Lumpur.
Jin, T., & Liu, L. (2008). The Wnt signaling pathway effector TCF7L2 and type 2 diabetes mellitus. Molecular Endocrinology, 22(11), 2383–2392. https://doi.org/10.1210/me.2008-0135
Kariadi, & Sri Hartini KS. (2009). Diabetes? Siapa Takut!! Panduan Lengkap Untuk Diabetisi, Keluarganya, dan Profesional Medis. Bandung: PT. Mizan Pustaka.
Radha, V., & Mohan, V. (2007). Genetic predisposition to type 2 diabetes among Asian Indians. The Indian Journal of Medical Research, 125(3), 259–274.
Sale, M., Smith, S. G., Mychaleckyj, J. C., Keene, K. L., Langefeld, C. D., Leak, T. S., … Freedman, B. I. (2007). Gene Are Associated With Type 2 Diabetes in an African-American Population Enriched for Nephropathy. October, 56(October), 2638–2642. https://doi.org/10.2337/db07-0012.Additional
Soelistijo, S., Novida, H., Rudijanto, A., Soewondo, P., Suastika, K., Manaf, A., … Soetedjo, N. (2015). Konsesus Pengelolaan Dan Pencegahan Diabetes Melitus Tipe2 Di Indonesia 2015. In Perkeni. Retrieved from https://www.google.com/url?sa=t&source=web&rct=j&url=https://pbperkeni.or.id/wp-content/uploads/2019/01/4.-Konsensus-Pengelolaan-dan-Pencegahan-Diabetes-melitus-tipe-2-di-Indonesia-PERKENI-2015.pdf&ved=2ahUKEwjy8KOs8cfoAhXCb30KHQb1Ck0QFjADegQIBhAB&usg=AOv
Syamsurizal, & Kadri, H. (2018). Genotyping SNP Rs12255372 TCF7L2 Gene Using Three-Primer ARMS-PCR for Detection T2DM n Indonesian Batak Ethnic. Journal of Physics: Conference Series, 1040(1). https://doi.org/10.1088/1742-6596/1040/1/012003
Syamsurizal, S., Handayani, D., Kadri, H., & Badriyya, E. (2019). Genotyping SNP rs7903146 TCF7L2 gene for detection T2DM in Indonesian melayu ethnic Genotyping SNP rs7903146 TCF7L2 gene for detection T2DM in Indonesian melayu ethnic. Journal of Physics: Conference Series, 1317, 1–8. https://doi.org/10.1088/1742-6596/1317/1/012090
Syamsurizal, S., Yanwirasti, Manaf, A., & Jamsari. (2014). Konstruksi Primer untuk Deteksi SNP rs12255372 pada Gen Transcription Factor 7 Like 2 (TCF7L2) Penyebab Diabetes Melitus Tipe-2 dengan Metode Amplification Refractory Mutation System (ARMS)– PCR. Prosiding Seminar Nasional Dan Wordshop Perkembangan Terkini Sains Farmasi Klinik 3. Padang.
Tama, B. A., F.S., R., & Hermansyah. (2011). An early detection method of type-2 diabetes mellitus in public hospital. TELKOMNIKA, 9(2), 287–294.
Votsi, C., Toufexis, C., Michailidou, K., Antoniades, A., Skordis, N., Karaolis, M., … Christodoulou, K. (2017). Type 2 Diabetes Susceptibility in the Greek-Cypriot Population: Replication of Associations with TCF7L2, FTO, HHEX, SLC30A8 and IGF2BP2 Polymorphisms. Genes, 8(1). https://doi.org/10.3390/genes8010016
World Health Organization. (2016). Global Report on Diabetes. France.
Yao, D. D., Yang, L., Wang, Y., Liu, C., Wei, Y. J., Jia, X. B., … Shu, L. (2015). Geniposide promotes beta-cell regeneration and survival through regulating β-catenin/TCF7L2 pathway. Cell Death and Disease, 6(5), e1746-13. https://doi.org/10.1038/cddis.2015.107
Yusuf, Z. K. (2010). Polymerase Chain Reaction (PCR). Saintek, 5(6), 1–6.
Yuwono, T. (2006). Teori dan Aplikasi Polymerase Chain Reaktion. Yogyakarta: andi.