The Growth of Alocasia macrorrhiza (L) Schott Variegata Roots on Murashige and Skoog Medium Supplemented 6-Benzyl Amino Purine (BAP) and Kinetin
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Alocasia, Variegata, BAP, Root
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Abstract
The sente variegata plant (Alocasia macrorrhiza variegata) is a species of ornamental taro. The unique leaf color of sente variegata, which is a combination of white and green, attracts ornamental plant lovers for hunting or collecting. There has been no cultivation effort from the ornamental plant suppliers and traders for multiplication purposes until now. Sente variegata is obtained directly from the forest. This activity can cause extinction. Micropropagation efforts using plant tissue culture techniques are needed for commercial and sustainability issues. This study aimed to determine the effect of 6-Benzyl Amino Purine (BAP) and kinetin on the root formation and growth as one step in the shoot multiplication of sente variegata bulb explants. Variations in BAP and kinetin concentrations used separately in this study were 0, 2, 5, and 10 mg. L-1. The results showed that the application of BAP and Kinetin significantly affected the root growth of sente Variegata. Finally, kinetin 10 mg L-1 is the concentration and type of cytokinins recommended for the formation and root growth of sente variegate.
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The Growth of Alocasia macrorrhiza (L) Schott Variegata Roots on Murashige and Skoog Medium Supplemented 6-Benzyl Amino Purine (BAP) and Kinetin. (2023). Jurnal Biota, 9(1), 8-14. https://doi.org/10.19109/Biota.v9i1.13904
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How to Cite
The Growth of Alocasia macrorrhiza (L) Schott Variegata Roots on Murashige and Skoog Medium Supplemented 6-Benzyl Amino Purine (BAP) and Kinetin. (2023). Jurnal Biota, 9(1), 8-14. https://doi.org/10.19109/Biota.v9i1.13904
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Asih, N. P. S., Hendriyani, E., & Tihurua, E. F. (2022). Morphological and Anatomical Variations among Alocasia alba Schott Accessions in Bali Botanic Garden. Journal of Tropical Biodiversity and Biotechnology, 7(1), 1–12. https://doi.org/10.22146/JTBB.66823
Bhaswatimayee, M., Rosanara, B., & Durga Prasad, B. (2021). Biotechnological Approaches for In-Vitro Propagation of a Valuable Medicinal Plant - Vitex Negundo L. International Journal of Pharma and Bio Sciences, 12(3), B-126-132. https://doi.org/10.22376/ijpbs.2021.12.3.b126-132
Dalton, S. J. (2020). A reformulation of Murashige and Skoog medium (WPBS medium) improves embryogenesis, morphogenesis and transformation efficiency in temperate and tropical grasses and cereals. Plant Cell, Tissue and Organ Culture, 141(2), 257–273. https://doi.org/10.1007/s11240-020-01784-8
Fay, M. F. (2018). Orchid conservation: how can we meet the challenges in the twenty-first century? Botanical Studies, 59(16). https://doi.org/10.1186/s40529-018-0232-z
Heriansyah, P. (2019). Multiplikasi Embrio Somatis Tanaman Anggrek (Dendrobium Sp) dengan Pemberian Kinetin dan Sukrosa Secara In-Vitro. Jurnal Ilmiah Pertanian, 15(2), 67–78. https://doi.org/10.31849/jip.v15i2.1974
Hesami, M., Daneshvar, M. H., Yoosefzadeh-Najafabadi, M., & Alizadeh, M. (2018). Effect of plant growth regulators on indirect shoot organogenesis of Ficus religiosa through seedling derived petiole segments. Journal of Genetic Engineering & Biotechnology, 16(1), 180. https://doi.org/10.1016/J.JGEB.2017.11.001
Justamante, M. S., Mhimdi, M., Molina-Pérez, M., Albacete, A., Moreno, M. Á., Mataix, I., & Pérez-Pérez, J. M. (2022). Effects of Auxin (Indole-3-butyric Acid) on Adventitious Root Formation in Peach-Based Prunus Rootstocks. Plants, 11(7). https://doi.org/10.3390/plants11070913
Karjadi, & Buchory. (2007). Pengaruh NAA Dan BAP Terhadap Pertumbuhan Jaringan Meristem Bawang Putih Pada Media B5. Jurnal Hortikultura, 17(3).
Mahadi, I. (2017). Propagasi In Vitro Anggrek (Dendrobium phalaenopsis Fitzg) terhadap Pemberian Hormon IBA dan Kinetin. Jurnal Agroteknologi, 7(1), 15–18. https://doi.org/10.24014/ja.v7i1.2244
Masykuroh, A., & Puspasari, H. (2022). Aktivitas Anti Bakteri Nano Partikel Perak (NPP) Hasil Biosintesis Menggunakan Ekstrak Keladi Sarawak Alocasia macrorrhizos terhadap Staphylococcus aureus dan Escherichia coli. Bioma : Jurnal Biologi Makassar, 7(1), 76–85. https://doi.org/10.20956/bioma.v7i1.19350
Mawaddah, S. K., Saputro, N. W., & Lestari, A. (2021). Pemberian Naphthalene Acetic Acid (NAA) dan Kinetin Terhadap Multiplikasi Tunas Tanaman Jahe (Globba leucantha var. bicolor Holttum) pada Kultur In Vitro. Bioma : Berkala Ilmiah Biologi, 23(1), 43–50. https://doi.org/10.14710/bioma.23.1.43-50
Nehnevajova, E., Ramireddy, E., Stolz, A., Gerdemann-Knörck, M., Novák, O., Strnad, M., & Schmülling, T. (2019). Root enhancement in cytokinin-deficient oilseed rape causes leaf mineral enrichment, increases the chlorophyll concentration under nutrient limitation and enhances the phytoremediation capacity. BMC Plant Biology, 19(83). https://doi.org/10.1186/s12870-019-1657-6
Obaid, A. A. (2018). Increasing of Cumarine And Caffic Acid Production From Apomixis Embryo of Citrus limon L. Brum F. and Citrus aurantifolia (Swingle) In Vitro. Thi-Qar University Journal for Agricultural Researches, 7(1), 55–70.
Oseni, O. M., Pande, V., & Nailwal, T. K. (2018). A Review on Plant Tissue Culture, A Technique for Propagation and Conservation of Endangered Plant Species. International Journal of Current Microbiology and Applied Sciences, 7(07), : 3778-3786. https://doi.org/10.20546/ijcmas.2018.707.438
Rishu, S., Shailesh Kumar, S., Saurabh Kumar, S., Sanjay, S., & Sonam. (2019). Cytokinin-a potential plant growth regulator for strawberry (Fragaria X ananassa Duch.) production. In Research Journal of Chemistry and Environment (Vol. 23, Issue 5). https://doi.org/10.37637/ab.v4i3.754
Saravia-Castillo, G., Tapia y Figueroa, L., & Borjas-Ventura, R. (2022). Auxins and Cytokinins elicit a differentiated response in the formation of shoots and roots in Cattleya maxima Lindl and Phalaenopsis amabilis (L) Blume. Scientia Agropecuaria, 13(1), 63–69. https://doi.org/10.17268/sci.agropecu.2022.006
Sjahril, R., Haring, F., Rukka, R. M., & ... (2019). Perbenihan Kultur Jaringan Anggrek Pada Teaching Industry Universitas Hasanuddin. Jurnal Dinamika …, 4(2), 146–156. https://doi.org/10.20956/jdp.v4i2.7415
Soumare, A., Diédhiou, A. G., Arora, N. K., Tawfeeq Al-Ani, L. K., Ngom, M., Fall, S., Hafidi, M., Ouhdouch, Y., Kouisni, L., & Sy, M. O. (2021). Potential Role and Utilization of Plant Growth Promoting Microbes in Plant Tissue Culture. In Frontiers in Microbiology (Vol. 12, p. 649878). https://doi.org/10.3389/fmicb.2021.649878
Sulasiah, A., Tumilisar, C., & Lestaria, T. (2015). Pengaruh Pemberian Jenis dan Konsentrasi Auksin Terhadap Induksi Perakaran Pada Tunas Dendrobium sp Secara In Vitro. BIOMA, 11(2). https://doi.org/10.21009/bioma11(2).5
Williams, S. J., Gale, S. W., Hinsley, A., Gao, J., & St. John, F. A. V. (2018). Using consumer preferences to characterize the trade of wild‐collected ornamental orchids in China. Conservation Letters, 11(5), 1–8. https://doi.org/10.1111/CONL.12569
Yachya, A., Fatiqin, A., Windi Lestari, R., Novita Fitriah, U., Decenly., Opi Anggoro, R., (2022). Studi Budidaya Jamur Kuping (Auricularia auricula) dengan Variasi Jenis Substrat dan Konsentrasi Supplemen. Journal of Biotropical Research and Nature Technology, 1(1), 21–28. https://doi.org/10.36873/BORNEO.V1I1.7329
Yachya, A., Manuhara, Y. S. W., & Novi, A. (2020). Impact of IBA and Ethephon Combination on Root Biomass Production of Javanese Ginseng ( Talinum paniculatum Gaertn ) Cuttings under Aeroponic System. Sysrevfarm, 11(7), 507–514. doi:10.31838/srp.2020.7.74
Yulia, E., Baiti, N., Handayani, R. S., & Nilahayati, N. (2020). Respon Pemberian Beberapa Konsentrasi BAP dan IAA terhadap Pertumbuhan Sub-Kultur Anggrek Cymbidium (Cymbidium finlaysonianum Lindl.) secara In-Vitro. Jurnal Agrium, 17(2). https://doi.org/10.29103/agrium.v17i2.5870
Zulkifli, Z., & Sari, P. L. (2019). Pengaruh Konsentrasi Bayclin pada Pencucian II dan BAP pada Media MS terhadap Pertumbuhan Eksplan Tanaman Pisang Klutuk (Musa paradisiaca. L) Secara In Vitro. Dinamika Pertanian, 33(2). https://doi.org/10.25299/dp.2017.vol33(2).3829