The Growth of Alocasia macrorrhiza Variegata Roots on Murashige and Skoog medium supplemented 6-Benzyl Amino Purine (BAP) and Kinetin
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Alocasia Variegata BAP Kinetin Root
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The Sente variegata plant (Alocasia macrorrchiza) is a species of gummy Taro. This plant is an ornamental plant that is in great demand because of the uniqueness of its leaves which have a combination of green and white. So far, Sente Variegata plants are obtained directly from the forest and there has been no cultivation effort from flower growers. Direct exploitation of a declining forest has the potential to cause extinction. Because of that. To meet the availability of seeds, it is necessary to have a propagation business with tissue culture techniques. The success factor of salat plant tissue culture is the presence of hormones used such as BAP and Kinetin. The purpose of this study is to determine the effect of 6-Benzyl Amino Purine (BAP) and Kinetin on the formation and growth of Roots which is an advanced stage of the multiplication stage of Sente Variegata seed explant shoots. This study is an experimental study using a complete randomized design (RAL) with 2 factors. The first factor is the type of hormone that is BAP and kinetin while the second factor is the variation of hormone concentrations of 0, 2, 5, and 10 mg.L-1 . Data analysis using the ANOVA test. followed by the Tukey test. The results showed that BAP and kinetin had a significant effect on growth (emergence time and Root Length). The best concentration of Kinetin hormone for root growth in Sente Variegata (Alocasia mycorrhiza) is Kinetin 10 mg.L-1
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The Growth of Alocasia macrorrhiza Variegata Roots on Murashige and Skoog medium supplemented 6-Benzyl Amino Purine (BAP) and Kinetin. (2023). Biota, 9(1), 8-14. https://doi.org/10.19109/Biota.v9i1.13904
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Cara Mengutip
The Growth of Alocasia macrorrhiza Variegata Roots on Murashige and Skoog medium supplemented 6-Benzyl Amino Purine (BAP) and Kinetin. (2023). Biota, 9(1), 8-14. https://doi.org/10.19109/Biota.v9i1.13904
Referensi
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Saravia-Castillo, G., Tapia y Figueroa, L., & Borjas-Ventura, R. (2022). Auxins and Cytokinins elicit a differentiated response in the formation of shoots and roots in Cattleya maxima Lindl and Phalaenopsis amabilis (L) Blume. Scientia Agropecuaria, 13(1), 63–69. https://doi.org/10.17268/sci.agropecu.2022.006
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Anjelia, S., Slamet, S., Wirasti, W., & Pambudi, D. B. (2021). Uji Aktivitas Mukolittik Ekstrak Etanol Daun Talas Senthe (Alocasia Macrorrhiza (L) Schott). Jurnal Ilmiah JOPHUS : Journal Of Pharmacy UMUS, 3(01), 37–45. https://doi.org/10.46772/jophus.v3i01.500
Asih, N. P. S., Hendriyani, E., & Tihurua, E. F. (2022). Morphological and Anatomical Variations among Alocasia alba Schott Accessions in Bali Botanic Garden. Journal of Tropical Biodiversity and Biotechnology, 7(1), 1–12. https://doi.org/10.22146/JTBB.66823
Bhaswatimayee, M., Rosanara, B., & Durga Prasad, B. (2021). Biotechnological Approaches for In-Vitro Propagation of a Valuable Medicinal Plant - Vitex Negundo L. International Journal of Pharma and Bio Sciences, 12(3), B-126-132. https://doi.org/10.22376/ijpbs.2021.12.3.b126-132
Dalton, S. J. (2020). A reformulation of Murashige and Skoog medium (WPBS medium) improves embryogenesis, morphogenesis and transformation efficiency in temperate and tropical grasses and cereals. Plant Cell, Tissue and Organ Culture, 141(2), 257–273. https://doi.org/10.1007/s11240-020-01784-8
Fay, M. F. (2018). Orchid conservation: how can we meet the challenges in the twenty-first century? Botanical Studies, 59(16). https://doi.org/10.1186/s40529-018-0232-z
Heriansyah, P. (2019). Multiplikasi Embrio Somatis Tanaman Anggrek (Dendrobium Sp) dengan Pemberian Kinetin dan Sukrosa Secara In-Vitro. Jurnal Ilmiah Pertanian, 15(2), 67–78. https://doi.org/10.31849/jip.v15i2.1974
Hesami, M., Daneshvar, M. H., Yoosefzadeh-Najafabadi, M., & Alizadeh, M. (2018). Effect of plant growth regulators on indirect shoot organogenesis of Ficus religiosa through seedling derived petiole segments. Journal of Genetic Engineering & Biotechnology, 16(1), 180. https://doi.org/10.1016/J.JGEB.2017.11.001
Justamante, M. S., Mhimdi, M., Molina-Pérez, M., Albacete, A., Moreno, M. Á., Mataix, I., & Pérez-Pérez, J. M. (2022). Effects of Auxin (Indole-3-butyric Acid) on Adventitious Root Formation in Peach-Based Prunus Rootstocks. Plants, 11(7). https://doi.org/10.3390/plants11070913
Karjadi, & Buchory. (2007). Pengaruh NAA Dan BAP Terhadap Pertumbuhan Jaringan Meristem Bawang Putih Pada Media B5. Jurnal Hortikultura, 17(3).
Mahadi, I. (2017). Propagasi In Vitro Anggrek (Dendrobium phalaenopsis Fitzg) terhadap Pemberian Hormon IBA dan Kinetin. Jurnal Agroteknologi, 7(1), 15–18. https://doi.org/10.24014/ja.v7i1.2244
Masykuroh, A., & Puspasari, H. (2022). Aktivitas Anti Bakteri Nano Partikel Perak (NPP) Hasil Biosintesis Menggunakan Ekstrak Keladi Sarawak Alocasia macrorrhizos terhadap Staphylococcus aureus dan Escherichia coli. Bioma : Jurnal Biologi Makassar, 7(1), 76–85. https://doi.org/10.20956/bioma.v7i1.19350
Mawaddah, S. K., Saputro, N. W., & Lestari, A. (2021). Pemberian Naphthalene Acetic Acid (NAA) dan Kinetin Terhadap Multiplikasi Tunas Tanaman Jahe (Globba leucantha var. bicolor Holttum) pada Kultur In Vitro. Bioma : Berkala Ilmiah Biologi, 23(1), 43–50. https://doi.org/10.14710/bioma.23.1.43-50
Nehnevajova, E., Ramireddy, E., Stolz, A., Gerdemann-Knörck, M., Novák, O., Strnad, M., & Schmülling, T. (2019). Root enhancement in cytokinin-deficient oilseed rape causes leaf mineral enrichment, increases the chlorophyll concentration under nutrient limitation and enhances the phytoremediation capacity. BMC Plant Biology, 19(83). https://doi.org/10.1186/s12870-019-1657-6
Obaid, A. A. (2018). Increasing of Cumarine And Caffic Acid Production From Apomixis Embryo of Citrus limon L. Brum F. and Citrus aurantifolia (Swingle) In Vitro. Thi-Qar University Journal for Agricultural Researches, 7(1), 55–70.
Oseni, O. M., Pande, V., & Nailwal, T. K. (2018). A Review on Plant Tissue Culture, A Technique for Propagation and Conservation of Endangered Plant Species. International Journal of Current Microbiology and Applied Sciences, 7(07), : 3778-3786. https://doi.org/10.20546/ijcmas.2018.707.438
Rishu, S., Shailesh Kumar, S., Saurabh Kumar, S., Sanjay, S., & Sonam. (2019). Cytokinin-a potential plant growth regulator for strawberry (Fragaria X ananassa Duch.) production. In Research Journal of Chemistry and Environment (Vol. 23, Issue 5). https://doi.org/10.37637/ab.v4i3.754
Saravia-Castillo, G., Tapia y Figueroa, L., & Borjas-Ventura, R. (2022). Auxins and Cytokinins elicit a differentiated response in the formation of shoots and roots in Cattleya maxima Lindl and Phalaenopsis amabilis (L) Blume. Scientia Agropecuaria, 13(1), 63–69. https://doi.org/10.17268/sci.agropecu.2022.006
Sjahril, R., Haring, F., Rukka, R. M., & ... (2019). Perbenihan Kultur Jaringan Anggrek Pada Teaching Industry Universitas Hasanuddin. Jurnal Dinamika …, 4(2), 146–156. https://doi.org/10.20956/jdp.v4i2.7415
Soumare, A., Diédhiou, A. G., Arora, N. K., Tawfeeq Al-Ani, L. K., Ngom, M., Fall, S., Hafidi, M., Ouhdouch, Y., Kouisni, L., & Sy, M. O. (2021). Potential Role and Utilization of Plant Growth Promoting Microbes in Plant Tissue Culture. In Frontiers in Microbiology (Vol. 12, p. 649878). https://doi.org/10.3389/fmicb.2021.649878
Sulasiah, A., Tumilisar, C., & Lestaria, T. (2015). Pengaruh Pemberian Jenis dan Konsentrasi Auksin Terhadap Induksi Perakaran Pada Tunas Dendrobium sp Secara In Vitro. BIOMA, 11(2). https://doi.org/10.21009/bioma11(2).5
Williams, S. J., Gale, S. W., Hinsley, A., Gao, J., & St. John, F. A. V. (2018). Using consumer preferences to characterize the trade of wild‐collected ornamental orchids in China. Conservation Letters, 11(5), 1–8. https://doi.org/10.1111/CONL.12569
Yachya, A., Fatiqin, A., Windi Lestari, R., Novita Fitriah, U., Decenly., Opi Anggoro, R., (2022). Studi Budidaya Jamur Kuping (Auricularia auricula) dengan Variasi Jenis Substrat dan Konsentrasi Supplemen. Journal of Biotropical Research and Nature Technology, 1(1), 21–28. https://doi.org/10.36873/BORNEO.V1I1.7329
Yachya, A., Manuhara, Y. S. W., & Novi, A. (2020). Impact of IBA and Ethephon Combination on Root Biomass Production of Javanese Ginseng ( Talinum paniculatum Gaertn ) Cuttings under Aeroponic System. Sysrevfarm, 11(7), 507–514. doi:10.31838/srp.2020.7.74
Yulia, E., Baiti, N., Handayani, R. S., & Nilahayati, N. (2020). Respon Pemberian Beberapa Konsentrasi BAP dan IAA terhadap Pertumbuhan Sub-Kultur Anggrek Cymbidium (Cymbidium finlaysonianum Lindl.) secara In-Vitro. Jurnal Agrium, 17(2). https://doi.org/10.29103/agrium.v17i2.5870
Zulkifli, Z., & Sari, P. L. (2019). Pengaruh Konsentrasi Bayclin pada Pencucian II dan BAP pada Media MS terhadap Pertumbuhan Eksplan Tanaman Pisang Klutuk (Musa paradisiaca. L) Secara In Vitro. Dinamika Pertanian, 33(2). https://doi.org/10.25299/dp.2017.vol33(2).3829