Endophytic Fungi in Cabbage Roots: Diversity and Antagonistic effects on Rhizoctonia solani
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Published:
Jan 18, 2024
Keywords:
Colonization frequency, in Vitro, Grass rhizosphere, Manure
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Abstract
Root endophytic fungi that living inside the plant roots without causing any symptom, basically is part of microorganisms in the rhizosphere or soil. Considering that, the objective of this study was to examine the effect of growth media on the occurrence and variabilities of culturable endophytic fungi in cabbage roots. The growth media examined were soil from pine forest, rhizosphere of cogon grass and elephant grass, inceptisol soil mixed with goat manure, compost or vermicompost (1:1, v/v). Fungal isolates obtained were examined their effect on the growth of cabbage seedlings and their abilities to inhibit the growth of fungal pathogen Rhizoctonia solani in vitro. The results showed that the growth media influenced the colonization and variabilities of fungal endophytes isolated from cabbage roots. The media supporting better colonization and variabilities of fungal endophytes was soil mixed with goat manure (1:1, v/v). Among 12 isolates obtained, three isolates (PK-2, PK-4 and PK-5 isolates) tended to improve the growth of cabbage seedlings. There were also three isolates (PK-1, PK-2 and TH-1) inhibited the growth of R. solani in vitro by 56.7% -64.7%.
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Endophytic Fungi in Cabbage Roots: Diversity and Antagonistic effects on Rhizoctonia solani. (2024). Jurnal Biota, 10(1), 62-72. https://doi.org/10.19109/Biota.v10i1.20134
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How to Cite
Endophytic Fungi in Cabbage Roots: Diversity and Antagonistic effects on Rhizoctonia solani. (2024). Jurnal Biota, 10(1), 62-72. https://doi.org/10.19109/Biota.v10i1.20134
References
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El Mansy, S. M., Nouh F. A. A., Mousa M. K., & Abdel-Azeem A.M. (2020) Endophytic fungi: diversity, abundance, and plant growth-promoting attributes. In: Yadav A., Mishra S., Kour D., Yadav N., Kumar A. (eds), Agriculturally important fungi for sustainable agriculture. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-030-45971-0_2
Erper, I., Ozer, G., Kalendar, R., Avci, S., Yildirim, E., Alkan, M., & Turkkan, M. (2021). Genetic Diversity and Pathogenicity of Rhizoctonia spp. Isolates Associated with Red Cabbage in Samsun (Turkey). Journal of Fungi, 7(3), 234. https://doi.org/10.3390/jof7030234
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Istifadah, N., Astriani, M., Sunarto, T. (2016). The potential of fungal endophytes from potato root and tubers to inhibit potato cyst nematode (Globodera Rostochiensis). Proceeding of International Conference on Biosciences, 10-17.
Istifadah, N. & Sari, I. P. (2017). Efek jamur endofit asal daun dan akar kacang tanah terhadap pertumbuhan dan penghambatan patogen inangnya. Jurnal Mikologi Indonesia, 1 (2), 60-69.
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Kracmarova M., Karpiskova J., Uhlik O., Strejcek M., Szakova J., Balik J., Demnerova K., Stiborova H. (2020). Microbial communities in soils and endosphere of Solanum tuberosum L. and their response to long-term fertilization. Microorganisms. 8(9),1377. https://doi.org/10.3390/microorganisms8091377
Lacava, P. T and Azevedo, J. L. (2014). Biological control of insect-pest and diseases by endophytes. In V. C. Verma & A. C. Gange (Eds.), Advances in endophytic research, Springer India, https://doi.org/10.1007/978-81-322-1575-2_13
Latz, M.A.C. B. Jensen, B., Collinge, D. B & Jørgensen, H. J. L. (2018). Endophytic fungi as biocontrol agents: elucidating mechanisms in disease suppression. Plant Ecology & Diversity, https://doi.org/10.1080/17550874.2018.1534146
Manalu, J. N, Poernomo, B., Soekarno, W., Tondok, E.T., & Surono S. 2020. Isolation and capability of dark septate endophyte against mancozeb fungicide. Jurnal Ilmu Pertanian Indonesia, 25 (2), 193-198
Poveda J., Díaz-González S., Díaz-Urbano M., Velasco P., and Sacristán S. (2022) Fungal endophytes of Brassicaceae: Molecular interactions and crop benefits. Front. Plant Sci. 13, 932288. https://doi.org/10.3389/fpls.2022.932288
Qian, X, Li, H., Wang Y., Wu, B., Wu, M., Chen, L., Li, X., Zhang, Y., Wang, X., Shi, M., Zheng Y, Guo, L., & Zhang, D. (2019). Leaf and root endospheres harbor lower fungal diversity and less complex fungal co-occurrence patterns than rhizosphere. Frontier Microbiology 10, 1015. https://doi.org/10.3389/fmicb.2019.01015
Rigobelo, E.C. & Baron, N. C. (2021). Endophytic fungi: a tool for plant growth promotion and sustainable agriculture, Mycology, 1-16
https://doi.org/10.1080/21501203.2021.1945699
Sheoran, H. S., Phogat, V. K., Dahiya, R., & Gera, R. (2018). Long-term effect of organic and conventional farming practices on microbial biomass carbon, enzyme activities and microbial populations in different textured soils of Haryana State (India). Applied Ecology and Environmental Research, 16(3), 3669-3689
http://dx.doi.org/10.15666/aeer/1603_36693689
Sun, X. & Guo, L-D (2012). Endophytic fungal diversity: review of traditional and molecular techniques, Mycology, 3(1), 65-76.
Urbina, H., Breed, M. F., Zhao, W., Lakshmi Gurrala, K., Andersson, S. G. E., Ågren, J., et al. (2018). Specificity in Arabidopsis thaliana recruitment of root fungal communities from soil and rhizosphere. Fungal Biology. 122, 231–240. https://doi.org/10.1016/J.FUNBIO.2017.12.013
Watanabe, T. 2010. Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to Species, Third Edition, CRC Press, Boca Raton, Florida.
Xia Y., Sahib M. R, Amna, A, Opiyo S.O, Zhenzhen Zhao, Z & Gao, Y. G. (2019). Culturable endophytic fungal communities associated with plants in organic and conventional farming systems and their effects on plant growth, Nature, 9, 1669 | https://doi.org/10.1038/s41598-018-38230-x
Ajayi-Oyetunde, O.O & Bradley, C. A. (2018). Rhizoctonia solani: taxonomy, population biology and management of Rhizoctonia seedling disease of soybean. Plant Pathology, 67 (1), 3-17. https://doi.org/10.1111/ppa.12733
Baron, N.C. & Rigobelo E.C. (2021). Endophytic fungi: a tool for plant growth promotion and sustainable agriculture. Mycology, 13(1), 39-55. doi: 10.1080/21501203.2021.1945699.
Berthelot, C., Chalot, M., Leyval, C., & Blaudez, D. (2019). From darkness to light: emergence of the mysterious dark septate endophytes in plant growth promotion and stress alleviation. In Hodkinson, T. R., Doohan, F. M., Saunders, M. J., Murphy, B. R., (Eds.) Endophytes for a growing world (pp. 143–164), Cambridge, UK: Cambridge University Press:
Bokati, D., Herrera, J. & Poudel, R. (2016). Soil influences colonization of root-associated fungal endophyte communities of maize, wheat, and their progenitors. Journal of Mycology, 8062073, http://dx.doi.org/10.1155/2016/8062073
Busby, P. E., Ridout, M., & Newcombe, G. (2015). Fungal Endophytes: Modifiers of Plant Disease. Plant Molecular Biology, 90(6), 645–655. doi: 10.1007/s11103-015-0412-0
Card, S. D., Hume, D. E., Roodi, D., McGill, C. R., Millner, J. P., & Johnson, R. D. (2015). Beneficial endophytic microorganisms of Brassica – A review. Biological Control, 90, 102–112. https://doi.org/10.1016/j.biocontrol.2015.06.001
Chen, J., Akutse, K. S., Saqib, H. S. A., Wu, X., Yang, F., Xia, X., Gurr, & G. M. (2020). Fungal endophyte communities of crucifer crops are seasonally dynamic and structured by plant identity, plant tissue and environmental factors. Frontiers in Microbiology, 11. https://doi.org/10.3389/fmicb.2020.01519
Diene, O., Wang, W., & Narisawa, K. (2013) Pseudosigmoidea ibarakiensis sp. nov., a dark septate endophytic fungus from a cedar forest in Ibaraki, Japan. Microbes Environ., 28(3):381-387. https://doi.org/10.1264/jsme2.me13002
El Mansy, S. M., Nouh F. A. A., Mousa M. K., & Abdel-Azeem A.M. (2020) Endophytic fungi: diversity, abundance, and plant growth-promoting attributes. In: Yadav A., Mishra S., Kour D., Yadav N., Kumar A. (eds), Agriculturally important fungi for sustainable agriculture. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-030-45971-0_2
Erper, I., Ozer, G., Kalendar, R., Avci, S., Yildirim, E., Alkan, M., & Turkkan, M. (2021). Genetic Diversity and Pathogenicity of Rhizoctonia spp. Isolates Associated with Red Cabbage in Samsun (Turkey). Journal of Fungi, 7(3), 234. https://doi.org/10.3390/jof7030234
Hanafiah, K. A. 2012. Rancangan percobaan : Teori dan Aplikasi. Edisi ke-3. Raja Grafindo Persada, Jakarta,
Hardoim P. R., van Overbeek L. S, Berg G, Pirttilä A. M, Compant, S., Campisano A, Döring M, & Sessitsch A. (2015). The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial endophytes. Microbiology Molecular Biology Review, 79 (3), 294-307.
Istifadah, N., Astriani, M., Sunarto, T. (2016). The potential of fungal endophytes from potato root and tubers to inhibit potato cyst nematode (Globodera Rostochiensis). Proceeding of International Conference on Biosciences, 10-17.
Istifadah, N. & Sari, I. P. (2017). Efek jamur endofit asal daun dan akar kacang tanah terhadap pertumbuhan dan penghambatan patogen inangnya. Jurnal Mikologi Indonesia, 1 (2), 60-69.
Jha, P., Kaur, T., Chhabra, I., Panja, A. Paul, S. Kumar, V., & Malik, T. (2023). Endophytic fungi: hidden treasure chest of antimicrobial metabolites interrelationship of endophytes and metabolites. Front. Microbiol. 14 – 2023 https://doi.org/10.3389/fmicb.2023.1227830
Khastini, R., Ohta, H., & Narisawa, K. 2012. The role of a dark septate endophytic fungus, Veronaeopsis simplex Y34, in Fusarium disease suppression in Chinese cabbage, The Journal of Microbiology, 50 (4): 618-624
Kracmarova M., Karpiskova J., Uhlik O., Strejcek M., Szakova J., Balik J., Demnerova K., Stiborova H. (2020). Microbial communities in soils and endosphere of Solanum tuberosum L. and their response to long-term fertilization. Microorganisms. 8(9),1377. https://doi.org/10.3390/microorganisms8091377
Lacava, P. T and Azevedo, J. L. (2014). Biological control of insect-pest and diseases by endophytes. In V. C. Verma & A. C. Gange (Eds.), Advances in endophytic research, Springer India, https://doi.org/10.1007/978-81-322-1575-2_13
Latz, M.A.C. B. Jensen, B., Collinge, D. B & Jørgensen, H. J. L. (2018). Endophytic fungi as biocontrol agents: elucidating mechanisms in disease suppression. Plant Ecology & Diversity, https://doi.org/10.1080/17550874.2018.1534146
Manalu, J. N, Poernomo, B., Soekarno, W., Tondok, E.T., & Surono S. 2020. Isolation and capability of dark septate endophyte against mancozeb fungicide. Jurnal Ilmu Pertanian Indonesia, 25 (2), 193-198
Poveda J., Díaz-González S., Díaz-Urbano M., Velasco P., and Sacristán S. (2022) Fungal endophytes of Brassicaceae: Molecular interactions and crop benefits. Front. Plant Sci. 13, 932288. https://doi.org/10.3389/fpls.2022.932288
Qian, X, Li, H., Wang Y., Wu, B., Wu, M., Chen, L., Li, X., Zhang, Y., Wang, X., Shi, M., Zheng Y, Guo, L., & Zhang, D. (2019). Leaf and root endospheres harbor lower fungal diversity and less complex fungal co-occurrence patterns than rhizosphere. Frontier Microbiology 10, 1015. https://doi.org/10.3389/fmicb.2019.01015
Rigobelo, E.C. & Baron, N. C. (2021). Endophytic fungi: a tool for plant growth promotion and sustainable agriculture, Mycology, 1-16
https://doi.org/10.1080/21501203.2021.1945699
Sheoran, H. S., Phogat, V. K., Dahiya, R., & Gera, R. (2018). Long-term effect of organic and conventional farming practices on microbial biomass carbon, enzyme activities and microbial populations in different textured soils of Haryana State (India). Applied Ecology and Environmental Research, 16(3), 3669-3689
http://dx.doi.org/10.15666/aeer/1603_36693689
Sun, X. & Guo, L-D (2012). Endophytic fungal diversity: review of traditional and molecular techniques, Mycology, 3(1), 65-76.
Urbina, H., Breed, M. F., Zhao, W., Lakshmi Gurrala, K., Andersson, S. G. E., Ågren, J., et al. (2018). Specificity in Arabidopsis thaliana recruitment of root fungal communities from soil and rhizosphere. Fungal Biology. 122, 231–240. https://doi.org/10.1016/J.FUNBIO.2017.12.013
Watanabe, T. 2010. Pictorial Atlas of Soil and Seed Fungi: Morphologies of Cultured Fungi and Key to Species, Third Edition, CRC Press, Boca Raton, Florida.
Xia Y., Sahib M. R, Amna, A, Opiyo S.O, Zhenzhen Zhao, Z & Gao, Y. G. (2019). Culturable endophytic fungal communities associated with plants in organic and conventional farming systems and their effects on plant growth, Nature, 9, 1669 | https://doi.org/10.1038/s41598-018-38230-x