Study of the Efficacy of Different Concentrations of Alcohol-Based Hand Sanitizer in Preventing Pathogen Transmission Through Palms

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Published: Aug 30, 2024
DOI: https://doi.org/10.19109/biota.v10i2.23231
Keywords:
Antibacterial efficacy, Alcohol-based hand sanitizer, Transmission of pathogen, Palms

Main Article Content

Evy Ratnasari Ekawati
Department of Medical Laboratory Technology, Faculty of Health and Science, Universitas Maarif Hasyim Latif, Sidoarjo, Jawa Timur, Indonesia
https://orcid.org/0000-0003-0920-5419
Setyo Dwi Santoso
Department of Medical Laboratory Technology, Faculty of Health and Science, Universitas Maarif Hasyim Latif, Sidoarjo, Jawa Timur, Indonesia
https://orcid.org/0009-0003-1792-3311
Muhammad Sungging Pradana
Department of Medical Laboratory Technology, Faculty of Health and Science, Universitas Maarif Hasyim Latif, Sidoarjo, Jawa Timur, Indonesia
https://orcid.org/0009-0001-6744-4285
Andreas Putro Ragil Santoso
Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, Surabaya, Jawa Timur, Indonesia
https://orcid.org/0000-0001-8869-9212
Mufida Hanum
Student of Medical Laboratory Technology, Faculty of Health Science, Universitas Maarif Hasyim Latif, Sidoarjo, Jawa Timur, Indonesia
https://orcid.org/0009-0007-8133-8992
Intan Wahyunillah Zaidah Khoir
Student of Medical Laboratory Technology, Faculty of Health Science, Universitas Maarif Hasyim Latif, Sidoarjo, Jawa Timur, Indonesia
https://orcid.org/0009-0006-1113-6271
Rosyidatul Arifima
Student of Medical Laboratory Technology, Faculty of Health Science, Universitas Maarif Hasyim Latif, Sidoarjo, Jawa Timur, Indonesia
https://orcid.org/0009-0004-2589-5960

Abstract

An integral part of the human body is the hands, which most frequently come into contact with various items; they can be an intermediary for spreading infections from hands to food and potentially eaten by humans. Maintaining hand hygiene is the right strategy to avoid this spread, which includes using an alcohol-based hand sanitizer. This study examines the potential for varying concentrations of alcohol-based hand sanitizer to transmit pathogens through the palms. The form of research is a laboratory experiment with an appropriate research design. Test the antibacterial potential of variations in alcohol-based hand sanitizer concentrations of 40, 50, 60, 70, and 70% using well diffusion and dilution methods. The tests were done in quadruplicates. The test bacteria used are Escherichia coli, Enterotoxigenic Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus. The results of the research showed that of the four variations in the concentration of alcohol-based hand sanitizer, the bacteriostatic ability against Salmonella typhimurium, Pseudomonas aeruginosa, and Klebsiella pneumoniae bacteria was at a concentration of 40% to 80%. The bactericidal activity at a concentration of 80% was demonstrated by Escherichia coli, Enterotoxigenic Escherichia coli, and Staphylococcus aureus. Bactericidal activity was demonstrated against Escherichia coli, Enterotoxigenic Escherichia coli, and Staphylococcus aureus at an 80% concentration.

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Study of the Efficacy of Different Concentrations of Alcohol-Based Hand Sanitizer in Preventing Pathogen Transmission Through Palms. (2024). Jurnal Biota, 10(2), 168-181. https://doi.org/10.19109/biota.v10i2.23231
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Vol. 10 No. 2 (2024): Jurnal Biota 2024
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Author Biographies

Evy Ratnasari Ekawati, Department of Medical Laboratory Technology, Faculty of Health and Science, Universitas Maarif Hasyim Latif, Sidoarjo, Jawa Timur, Indonesia

Department of Medical Laboratory Technology, Faculty of Health and Science, UMAHA

Setyo Dwi Santoso, Department of Medical Laboratory Technology, Faculty of Health and Science, Universitas Maarif Hasyim Latif, Sidoarjo, Jawa Timur, Indonesia

Department of Medical Laboratory Technology, Faculty of Health and Science, UMAHA

Muhammad Sungging Pradana, Department of Medical Laboratory Technology, Faculty of Health and Science, Universitas Maarif Hasyim Latif, Sidoarjo, Jawa Timur, Indonesia

Department of Medical Laboratory Technology, Faculty of Health and Science, Universitas Maarif Hasyim Latif

Andreas Putro Ragil Santoso, Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, Surabaya, Jawa Timur, Indonesia

Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya

Intan Wahyunillah Zaidah Khoir, Student of Medical Laboratory Technology, Faculty of Health Science, Universitas Maarif Hasyim Latif, Sidoarjo, Jawa Timur, Indonesia

Student Faculty of Health Science, Universitas Maarif Hasyim Latif

Rosyidatul Arifima, Student of Medical Laboratory Technology, Faculty of Health Science, Universitas Maarif Hasyim Latif, Sidoarjo, Jawa Timur, Indonesia

Student of Faculty of Health Science, Universitas Maarif Hasyim Latif

How to Cite

Study of the Efficacy of Different Concentrations of Alcohol-Based Hand Sanitizer in Preventing Pathogen Transmission Through Palms. (2024). Jurnal Biota, 10(2), 168-181. https://doi.org/10.19109/biota.v10i2.23231

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