Exploration of Plastic Degrading Bacteria in the Musi River, PalembangCity

Article Sidebar

PDF
Published: Apr 26, 2025
DOI: https://doi.org/10.19109/biota.v0i0.27347
Keywords:
Bacillus; Bacteria; Biodegradation; Musi River; Plastic Pollution.

Main Article Content

Hary Widjajanti
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya, South Sumatra, Indonesia
https://orcid.org/0000-0001-5555-1537
Annisa Kemala Tasya
Graduate School of Sciences, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya, South Sumatra, Indonesia
Laila Hanum
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya, South Sumatra, Indonesia
https://orcid.org/0000-0003-4748-2417

Abstract

Musi River is a waterway in South Sumatra that faces increasing plastic pollution, threatening aquatic ecosystems and human life. This study aims to identify and evaluate plastic-degrading bacteria from Musi River using phenotypic and molecular approaches. The methods of this study include bacterial isolation, plastic degradation test, and Scanning Electron Microscopy (SEM) analysis were carried out to assess degradation efficiency and changes in surface morphology, phenotypic and molecular identification. The results showed that isolates S1I3, S1I5, and S2I1 had high plastic degradation potential, with levels of 38.03%, 34.73%, and 30.46%, respectively. SEM observations showed changes in surface morphology, including pores and cracks. Molecular identification confirmed that S1I3 was Bacillus proteolyticus (99.87%), while S1I5 and S2I1 matched Bacillus cereus (100%).

Downloads

Download data is not yet available.

Article Details

How to Cite
Exploration of Plastic Degrading Bacteria in the Musi River, PalembangCity. (2025). Jurnal Biota. https://doi.org/10.19109/biota.v0i0.27347
Issue
ACCEPTED MANUSCRIPT
Section
Artikel
Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors who publish with this journal agree to the following terms:
1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

How to Cite

Exploration of Plastic Degrading Bacteria in the Musi River, PalembangCity. (2025). Jurnal Biota. https://doi.org/10.19109/biota.v0i0.27347

References

K. P. T. Sianturi, B. Amin, and M. Galib, “Microplastic Distribution in Sediments in Coastal of Pariaman City, West Sumatera Province,” Asian J. Aquat. Sci., vol. 4, no. 1, pp. 73–79, 2021.

N. Evode, S. A. Qamar, M. Bilal, D. Barceló, and H. M. N. Iqbal, “Plastic waste and its management strategies for environmental sustainability,” Case Stud. Chem. Environ. Eng., vol. 4, no. September, 2021.

A. Supit, L. Tompodung, and S. Kumaat, “Mikroplastik sebagai Kontaminan Anyar dan Efek Toksiknya terhadap Kesehatan Microplastic as an Emerging Contaminant and its Toxic Effects on Health,” J. Kesehat., vol. 13, pp. 199–208, 2022.

T. van Emmerik and A. Schwarz, “Plastic debris in rivers,” Wiley Interdiscip. Rev. Water, vol. 7, no. 1, pp. 1–24, 2020.

A. Macali et al., “Episodic records of jellyfish ingestion of plastic items reveal a novel pathway for trophic transference of marine litter,” Sci. Rep., vol. 8, no. 1, pp. 1–5, 2018.

P. Agrawal and R. Kumar singh, “Breaking Down Of Polyethylene By Pseudomonas Species,” Int. J. Sci. Eng. Res. Vol., vol. 7, no. 3, pp. 124–127, 2016.

P. Lalrinfela, R. Vanlalsangi, K. Lalrinzuali, and P. J. Babu, “Microplastics_ Their effects on the environment, human health, and plant ecosystems,” Environ. Pollut. Manag., vol. 1, no. November, pp. 248–259, 2024.

N. Mohanan, Z. Montazer, P. K. Sharma, and D. B. Levin, “Microbial and Enzymatic Degradation of Synthetic Plastics,” Front. Microbiol., vol. 11, no. November, 2020.

A. P. Asiandu, A. Wahyudi, and S. W. Sari, “A Review: Plastics Waste Biodegradation Using Plastics-Degrading Bacteria,” J. Environ. Treat. Tech., vol. 9, no. 1, pp. 148–157, 2020.

D. R. Adhika, A. L. Anindya, V. V. Tanuwijaya, and H. Rachmawati, “Teknik Pengamatan Sampel Biologi Dan Non-Konduktif Menggunakan Scanning Electron Microscopy,” no. January, 2019.

N. S. Pangestu, A. Budiharjo, and M. I. Rukmi, “Isolasi, Identifikasi 16s rRNA dan Karakterisasi Morfologi Bakteri Pendegradasi Plastik Polietilen (PE),” J. Biol., vol. 5, no. 1, pp. 24–29, 2016.

A. Sriningsih and M. Shovitri, “15619-ID-potensi-isolat-bakteri-pseudomonas-sebagai-pendegradasi-plastik,” J. Sains dan Seni ITS, vol. 4, no. 2, pp. 67–70, 2015.

Rhezqy Furwati Jufri, “Microbial Isolation,” J. La Lifesci, vol. 01, no. 01, pp. 18–23, 2020.

N. I. . Undap, D. A. Sumilat, and R. Bara, “Antibacterial substances of sponges, Agelas tubulata and Phyllospongia sp., from Manado Bay, against the growth of several bacterial strains,” Aquat. Sci. Manag., vol. 5, no. 1, p. 23, 2019.

G. B. & P. K, “STUDY OF THE ENUMERATION OF TWELVE CLINICAL IMPORTANT BACTERIAL STANDARD,” vol. 6, no. 2, pp. 880–893, 2018.

C. Masi, G. Gemechu, and M. Tafesse, “Identification of Alkaline Protease- Producing Bacteria From Leather Industry Effluent,” Ann. Microbiol., vol. 71, no. 24, pp. 2–11, 2021.

D. A. Irza, I. Nyoman Ehrich Lister, S. Sihotang, and E. Fachrial, “Isolation, characterization, molecular identification of probiotic bacteria from meconium,” IOP Conf. Ser. Earth Environ. Sci., vol. 755, no. 1, 2021.

Auta, H. S., Emenike, C. U., and S. H. Jayanthi & Fauziah, “Growth kinetics and biodeterioration of polypropylene microplastics by Bacillus sp. And Rhodococcus sp. Isolated from mangrove sediment,” Mar. Pollut. Bull., vol. 127: 15–21, 2018.

Xiang, P., Yunfeng, Z., Ting, Z., Qian, W., and L. Changsong, Z., & Qiang, “A novel bacterial combination for effecient degradation of polystyrene microplastis.,” J. Hazard. Mater., vol. 458: 13185, 2023.

N. . Mulyaningsih, W. Fita, and E. W. Sri, “Rekomendasi Teknik Analisis Citra SEM dengan Menggunakan Free Software ImageJ,” J. Fis. dan Terap., vol. 5, no. 2, p. 6, 2020.

S. Anindita, M. Anwar, T. Taufiq, and T. D. Wahyuningsih, “Ketahanan Isolat Bakteri Asal Feses Bayi Terhadap Variasi Suhu dan pH,” Proceeding Heal. Archit., vol. 1, no. 1, pp. 978–602, 2017.

P. S. Gemilang, “Gangguan Kesehatan pada Masyarakat yang Disebabkan oleh Bakteri Mesofilik,” J. Pengabdi. Masy. Biol. dan Sains, vol. 2, no. 2, pp. 56–61, 2023.

D. Anwariani, “Pengaruh Air Limbah Domestik Terhadap Kualitas Sungai,” J. Tek. Lingkung., vol. 9, no. 6, pp. 1–6, 2019.

M. I. Filayani, “Uji Degradasi Plastik Polietilen Menggunakan Metode Kolom Winogradsky dengan Penambahan Lactobacillus bulgaricus dan Streptococcus thermopilus Polyethhylene Plastic Degradation Test Using the Winogradsky Column Method with Lactobacillus bulgaricus and Str,” J. lentera Bio, vol. 9, no. 2, pp. 153–157, 2020.

M. A. Syah, Y. Yaddi, and N. I. Ulfa, “Identifikasi Molekuler Bakteri Lipolitik Yang Diisolasi Dari Sedimen Mangrove Teluk Kendari,” vol. 11, no. 1, pp. 68–77, 2024.

G. Z. Muflih et al., “Pelatihan Canva Sebagai Implementasi Program Adaptasi Teknologi Pada Kurikulum Merdeka Untuk Siswa Smp Pgri 1,” vol. 5, pp. 427–432, 2024.

D. A. Sendjaya, I. R. Kardila, S. Lestari, and D. Kusumawaty, “Review: Potensi Bakteri Dari Saluran Pencernaan Ikan Sidat (Anguilla sp.) Sebagai Pendegradasi Sampah Plastik,” Indobiosains, vol. 3, no. 2, p. 18, 2021.

Z. Montazer, M. B. H. Najafi, and D. B. Levin, “Challenges with verifying microbial degradation of polyethylene,” Polymers (Basel)., vol. 12, no. 1, 2020.

Harsh Bhardwaj, Meenakshi Gupta, and Anurag Tiwari, “Communities of Microbial Enzymes Associated with Biodegradation of Plastics,” J. Polym. Environ., vol. 21, pp. 183–194, 2013.

I. E. Gilani, S. Sayadi, N. Zouari, and M. A. Al-Ghouti, “Plastic waste impact and biotechnology: Exploring polymer degradation, microbial role, and sustainable development implications,” Bioresour. Technol. Reports, vol. 24, no. May, p. 101606, 2023.

A. Yamashita et al., Harmonization of resting-state functional MRI data across multiple imaging sites via the separation of site differences into sampling bias and measurement bias, vol. 17, no. 4. 2019.

R. Restrepo-Florez, J. M., Restrepo, A., & Sirohi, “Microbial degradation and deterioration of polyethylene – A review,” Environ. Sci. Pollut. Res., vol. 21, pp. 13969–13983, 2014.

Dewi, R. and N. Sari, D. P., & Suryani, “Karakteristik fenotipik dan uji koagulase pada isolat bakteri genus Staphylococcus dari kulit mahasiswa Fakultas Kedokteran Universitas Udayana.,” J. Biomedik, vol. 9(2), pp. 85–90, 2017.

A. A. Akinsemolu, H. Onyeaka, S. Odion, and I. Adebanjo, “Exploring Bacillus subtilis: Ecology, biotechnological applications, and future prospects,” J. Basic Microbiol., vol. 64, no. 6, 2024.

A. K. R. Purba et al., “The impacts of deep surgical site infections on readmissions, length of stay, and costs: A matched case–control study conducted in an academic hospital in the netherlands,” Infect. Drug Resist., vol. 13, pp. 3365–3374, 2020.

D. Danso, J. Chow, and W. R. Streita, “Plastics: Environmental and biotechnological perspectives on microbial degradation,” Appl. Environ. Microbiol., vol. 85, no. 19, pp. 1–14, 2019.

R. Jabeen et al., “Medicinal potential of antimicrobial peptides from two plants against Bacillus cereus and Staphylococcus aureus,” Ital. J. Med., vol. 18, no. 1, 2024.

S. Noer, “Identifikasi Bakteri secara Molekular Menggunakan 16S rRNA,” EduBiologia Biol. Sci. Educ. J., vol. 1, no. 1, p. 1, 2021.

H. Mukadam, S. V. Gaikwad, N. N. Kutty, and V. D. Gaikwad, “Bioformulation of Bacillus proteolyticus MITWPUB1 and its biosurfactant to control the growth of phytopathogen Sclerotium rolfsii for the crop Brassica juncea var local, as a sustainable approach,” Front. Bioeng. Biotechnol., vol. 12, no. April, pp. 1–14, 2024.

W. Irawati, E. Lucky, Y. P. . Tumakaka, C. Immanuel, and E. B. Marvella, “Karakterisasi Bacillus cereus strain IrN yang memiliki sifat multiresistensi terhadap tembaga dan pewarna,” J. Biol. Udayana, vol. 26, no. 2, p. 248, 2022.

X. Y. Liu, Q. Hu, F. Xu, S. Y. Ding, and K. Zhu, “Characterization of bacillus cereus in dairy products in China,” Toxins (Basel)., vol. 12, no. 7, pp. 1–18, 2020.

W. A. Fietri, A. Rajak, and R. Sumarmin, “Analisis Filogenetik Familia Ikan Kerapu Serranidae Berdasarkan Penandaan Chytocrome Oxydase I (COI) dari Pasar Ikan Lokal di Indonesia,” J. Biol. Papua, vol. 13, no. 2, pp. 129–136, 2021.