Effect of Different Strenght of Medium on Germination and Seedling Growth of Tomato and Sucrose Effect on Biomass of Tomato Callus
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Published:
May 30, 2020
Keywords:
callus, hypocotyl, strenght of medium, sucrose, tomato
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Abstract
Bioactive compounds of plant cell culture extracts are currently being looked at for its potential for the development of nutraceutical products. Tomato callus is known to have cytoprotective activity. The availability for explants and its callus production are two important things. This paper reports the effect of different strengths of medium on seed germination and seedling growth of tomato cv. Permata and sucrose effects on their biomass callus. Seeds were grown on 4 kind of germination media. Hypocotyl were taken as explants for callus induction in MS medium supplemented by 2.0 mg/l BA and 0.2 mg/l NAA, 3% sucrose, and subcultured after 20 days on medium with 6 variations of sucrose. The highest values for tomato hypocotyl’s height were obtained from the ½ and ¼ MS, while the highest fresh weight was obtained from the MS 0. Both the fresh weight and dry weight of the callus increased along with increasing sucrose concentration. The explant age and biomass need to be a concern regarding what kind of callus that will be produced. Application of 5% sucrose concentration is recommended for callus production in this study.
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Effect of Different Strenght of Medium on Germination and Seedling Growth of Tomato and Sucrose Effect on Biomass of Tomato Callus. (2020). Jurnal Biota, 6(1), 12-18. https://doi.org/10.19109/Biota.v6i1.4184
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How to Cite
Effect of Different Strenght of Medium on Germination and Seedling Growth of Tomato and Sucrose Effect on Biomass of Tomato Callus. (2020). Jurnal Biota, 6(1), 12-18. https://doi.org/10.19109/Biota.v6i1.4184
References
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Riastri, A. (2019). Uji efek sitoprotektif ekstrak sel punca kecambah tomat (Solanum lycopersicum L.) dan analisis ekspresi sitokin TNF-Alpha pada sel Human Dermal Fibroblast Adult (HDFa) yang diinduksi sinar UV-B (Unpublished undergraduate thesis). Universitas Gadjah Mada, Yogyakarta, Indonesia. Sari, Y. P., Kusumawati, E., Saleh, C., Kustiawan, W., & Sukartiningsih, S. (2018). Effect of sucrose and plant growth regulators on callogenesis and preliminary secondary metabolic of different explant Myrmecodia tuberosa. Nusantara Bioscience, 10(3), 183-192. Schmid, D., Schurch, C., Blum, P., Belser, E., & Zulli, F. (2008). Plant stem cell extract for longevity of skin and hair. Journal Seifen Ole Fette Wachse, 134(5), 30-35. Shahnewaz, S., & Bari, M. A. (2004). Effect of concentration of sucrose on the frequency of callus induction and plant regeneration in anther culture of rice (Oryza sativa L.). Plant Tissue Culture, 14(1), 37-43. Skoog, F., & Miller, C. (1957). Chemical regulation of growth and organ formation in plant tissues cultured. Symposia of the Society for Experimental Biology, 11, 118-30. Smith, R. H. (2012). Plant tissue culture: techniques and experiments (3rd Ed.). London, England: Academic Press. Sumaryono, S., Muslihatin, W., & Ratnadewi, D. (2012). Effect of carbohydrate source on growth and performance of In Vitro sago palm (Metroxylon sagu Rottb.) plantlets. HAYATI Journal of Biosciences, 19(2), 88-92. VanderMolen, K. M., Raja, H. A., El-Elimat, T., & Oberlies, N. H. (2013). Evaluation of culture media for the production of secondary metabolites in a natural products screening program. AMB Express, 3(1), 1-7. Wojtania, A., Gabryszewska, E., & Podwyszyńska, M. (2011). The effect of growth regulators and sucrose concentration on in vitro propagation of Camellia japonica L. Propagation of Ornamental Plants, 11(4), 177-183. Yaseen, M., Ahmed, T., Abbasi, N. A., & Hafiz, I. A. (2009). In vitro shoot
proliferation competence of apple rootstocks M. 9 and M. 26 on different carbon sources. Pakistan Journal of Botany, 41(4), 1781-1795. Yildiz, M., Onde, S., & Ozgen, M. (2007). Sucrose effects on phenolic concentration and plant regeneration from sugarbeet leaf and petiole explants. Journal of Sugar Beet Research, 44(1), 1-15.