Optimization of pH on Enzymatic Activity of Eco-Enzyme Averrhoa bilimbi L. in Plaju District, South Sumatra
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Published:
Aug 11, 2023
Keywords:
Enzymatic activity, Averrhoa bilimbi L, Eco-enzyme, pH.
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Abstract
Enzymes are biocatalysts that have many benefits in the industry and the environment. Eco-enzymes are reported to have amylase, lipase, and protease activities. Enzymatic activity is strongly influenced by several factors, one of which is pH. This study aims to determine the optimum enzymatic activity (amylase, lipase and protease) of Averrhoa bilimbi L. fruit eco-enzymes with various pH treatments (5, 6, 7, 8). Enzymatic activity was measured using a spectrophotometric method. Amylase activity assay using starch as a substrate. Casein and para nitrophenol palmitate were used as substrates. Casein was used as a substrate in the protease activity assays. In the lipase activity assays, para nitrophenol palmitate was used as substrate. Data analysis results are presented in graphical and descriptive. Averrhoa bilimbi L. fruit eco-enzyme has a protein concentration of 0.459 mg/ml. The optimum activity of amylase at pH 5 was 11,713.871 U/mg. Optimum activity and protease activity occurred at pH 8 and 6 of 3.667 U/mg and 13,400.77 U/mg, respectively.
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Optimization of pH on Enzymatic Activity of Eco-Enzyme Averrhoa bilimbi L. in Plaju District, South Sumatra. (2023). Jurnal Biota, 9(2), 72-79. https://doi.org/10.19109/Biota.v9i2.16460
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How to Cite
Optimization of pH on Enzymatic Activity of Eco-Enzyme Averrhoa bilimbi L. in Plaju District, South Sumatra. (2023). Jurnal Biota, 9(2), 72-79. https://doi.org/10.19109/Biota.v9i2.16460
References
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Hemalatha, M., & Visantini, P. (2020). Potential Use of Eco-enzyme for the Treatment of Metal Based Effluent. IOP Conf. Series: Materials Science and Engineering, 716, 1-6. https://doi:10.1088/1757-899X/716/1/012016
Istia’nah, D., Utami, U., & Barizi, A. (2020). Karakterisasi Enzim Amilase dari Bakteri Bacillus megaterium pada Variasi Suhu, pH dan Konsentrasi Substrat. Jurnal Riset Biologi Dan Aplikasinya, 2(1), 11. https://doi.org/10.26740/jrba.v2n1.p11-17
Jadid, N., Jannah, A. L., Handiar, B. P. W. P., Nurhidayati, T., Purwani, K. I., Ermavitalin, D., & Navastara, A. M. (2022). Aplikasi Eco Enzyme sebagai Bahan Pembuatan Sabun Antiseptik. Sewagati, 6(1), 69-75.
Kusumaningrum, A., Gunam, I. B. W., & Wijaya, I. M. M. (2019). Optimization of Temperature and pH on Endoglucanase Enzyme Activity Using Response Surface Methodology (RSM). Jurnal Rekayasa dan Manajemen Agroindustri, 7(2), 243-253.
Larasati, D., Astuti, A. P., & Maharani, E. T. (2020). Uji Organoleptik Eco-Enzyme dari Limbah Kulit Buah (Studi Kasus di Kota Semarang). Seminar Nasional Edusainstek, 278–283. https://doi.org/10.31539/bioedusains.v5i1.3387
Layly, I. R., & Wiguna, N. O. (2016). Studi Potensi Lipase Alcaligenes faecalis Untuk Aplikasi Biodeterjen. Jurnal Bioteknologi & Biosains Indonesia, 3(2), 66–71.
Lestari, P., Raharjo, T. K., Matsjeh, S. and Haryadi. W. (2016). Partial Purification and Biochemical Characterization of Extracellular Lipase from Azospirillum sp. JG3 Bacteria. Advances of Science and Technology for Society. AIP Conf. Proc.
Nangin, D., & Sutrisno, A. (2015). Enzim Amilase Pemecah Pati Mentah dari Mikroba : Kajian Pustaka. Jurnal Pangan Dan Agroindustri, 3(3), 1032–1039.
Nazim, F., & Meera, V. (2015). Use of Garbage Enzyme as a Low Cost Alternative Method for Treatment of Greywater - A review. Journal of Environmental Science and Engineering.
Nurdin, Nasihin, L., Herlina, N., Supartono, T., Kosasih, D., & Nurlaila, A. (2021). Pemanfaatan Sampah Organik Sebagai Biohandsanitizer dan Biodesinfektan Berbasis Eco-Community Untuk Mencegah Penyebaran Virus Corona. Jurnal Berdaya Mandiri, 3(2), 578–587.
Nurhamidah, Amida, N., Rohiat, S., & Elvinawati. (2021). Pengolahan Sampah Organik Menjadi Eco-Enzyme pada Level Rumah Tangga menuju Konsep Eco-Community. Andromeda, 1(2), 43–46.
Nurkhotimah, Yulianti, E., & Rakhmawati, A. (2017). The Influence of Temperature and pH Toward the Activity of the Phosphatase Enzyme from Thermophilic Bacteria of Gendol River Pasca Merapi Eruption. Jurnal Prodi Biologi, 6(8),465-471.
Pagarra, H., Hartati & Rachmawaty. (2021). Optimization of Protease Enzymes by Endophytic Bacteria from Roots of Karst Ecosystem Areas with Response Surface Methodology. Jurnal Sainsmat, 10(2),140-151.
Penitobe, T. (2021). Pengaruh Penambahan Enzim Protease Kasar (Crude Protease Enzyme) dari Daun Kelor (Moringga olivera) Terhadap Uji Organoleptik dan Kadar Asam Laurat Pada VCO (Virgin Coconut Oil). Skripsi, 3(April), 49–58.
Pratamadina, E., & Wikaningrum, T. (2022). Potensi Penggunaan Eco Enzyme pada Degradasi Deterjen dalam Air Limbah Domestik. Jurnal Serambi Engineering, 7(1), 2722–2728. https://doi.org/10.32672/jse.v7i1.3881
Purwanto, M. G. M. (2014). Perbandingan Analisa Kadar Protein Terlarut Dengan Berbagai Spektroskopi UV-Visible. Jurnal Ilmiah Sains dan Teknologi, 7(2), 64-71.
Rasit, N., Fern, L. H., & Ghani, W. A. W. A. K. (2019).
Rochyani, N., Utpalasari, R. L., & Dahliana, I. (2020). Analisis Hasil Konversi Eco Enzyme Menggunakan Nenas (Ananas comosus) dan Pepaya (Carica papaya L.). Jurnal Redoks, 5(2), 135–140.
Sari, E. M., & Fadhilah, T. M. (2021). Penentuan Kadar Protein Albumin dalam Sampel Brownies yang Diberikan Kepada Penderita Tuberkulosis. Chimica et Natura Acta, 9(2), 45-49. https://doi.org/10.24198/cna.v9.n2.34096
Soeka, Y. S., & Sulistiani, S. (2017). Karakterisasi Enzim Protease dari Bakteri Stenotrophomonas sp. Asal Gunung Bromo, Jawa Timur. LIPI, 16(2). https://doi.org/10.14203/beritabiologi.v16i2.2940
Soleha, S., & Retnaningrum, E. (2020). Optimization Extracellular Lipase Activity from Moraxella sp SBE01 for Hydrocarbons Nanoremediation. AIP Conference Proceedings 2260, 1 – 7. https://doi.org/10.1063/5.0015926.
Sumardi, Farisi S., Ekowati, C. N., & Diana, M. S. (2019). Aktivitas dan Karakterisasi Enzim Protease Isolat Bacillus sp. (UJ132) Secara Kualitatif dan Kuantitatif. Jurnal Riset Akuakultur, 14 (3), 193-199. http://ejournal-balitbang.kkp.go.id/index.php/jra
Sumarlin, L. O. (2008). Aktivitas Protease dari Bacillus circulans pada Media Pertumbuhan Dengan pH Tidak Terkontrol. Jurnal Kimia VALENSI, 1(2), 58–62. https://doi.org/10.15408/jkv.v1i2.215
Supriyatna, A., Amalia, D., Jauhari, A. A., & Holydaziah, D. (2015). Aktivitas Enzim Amilase, Lipase, dan Protease dari Larva Hermetia illucens yang Diberi Pakan Jerami Padi. Edisi Juli 2015, IX(2), 18–32.
Tazkiah, N. P., Rosahdi, T. D., & Supriadin, A. (2017). Isolasi dan Karakterisasi Enzim Amilase dari Biji Nangka (Artocarpus heterophillus). Al-Kimiya, 4(1), 17–22. https://doi.org/10.15575/ak.v4i1.5079
Tondais, J., Sombo, D. E., Lalenoh, B. A., Mappiratu, M., Adrian, A., & Cahyono, E. (2020). Ekstraksi Flavour dari Tepung Ikan Layang (Decapterus sp.) Menggunakan Enzim Protease Biduri (Calotropis gigantea). Jurnal FishtecH, 9(1), 6–12. https://doi.org/10.36706/fishtech.v9i1.11481
Wahjuni, S., Suarya, P., & Saputra, I. M. A. (2017). Isolasi Enzim Amilase dari Kecambah Biji Jagung Lokal Seraya (Zea mays L.) untuk Hidrolisis Pati. Jurnal Kimia, 11(2), 122–128. https://www.ptonline.com/articles/how-to-get-better-mfi-results
Zainudin & Kesumaningwati, R. (2022). Effect of Eco Enzyme on Heavy Metal Content in Ex-Coal Mining Land. Ziraa’ah, 47 (2), 154-161
Ariandi. (2016). Pengenalan Enzim Amilase (Alpha-Amylase) dan Reaksi Enzimatisnya Menghidrolisis Amilosa Pati Menjadi Glukosa. Jurnal Dinamika, 07(1), 74–82.
Arun, C., Sivashanmugam, P. (2015). Investigation of Biocatalytic Potential of Garbage Enzyme and Its Influence on Stabilization of Industrial Waste Activated Sludge. Process Safety and Environmental Protection, 94, 471-478. http://dx.doi.org/10.1016/j.psep.2014.10.008
Astra, I. K. B., Wijaya, M. A., Artanayasa, I. W., & Kardiawan, I. K. H. (2021). Pengolahan Sampah Organik Berbasis Eco Enzyme Sebagai Upaya Pembentukan Karakter Peduli Lingkungan Pemuda Di Kabupaten Buleleng. Proceeding Senadimas Undiksha 2021, 2065–2073. https://lppm.undiksha.ac.id/senadimas2021/prosiding/file/279.pdf
Budiyanto, C. W., Yasmin, A., Fitdaushi, A. N., Rizqia, A. Q. S. Z., Safitri, A. R., Anggraeni, D. N., Farhana, K. H., Alkatiri, M. Q., Perwira, Y. Y., & Pratama, Y. A. (2022). Mengubah Sampah Organik Menjadi Eco Enzym Multifungsi: Inovasi di Kawasan Urban. DEDIKASI: Community Service Reports, 4(1), 31–38. https://doi.org/10.20961/dedikasi.v4i1.55693
Engka, D. L., Kandou, J., Koapaha, T., & . (2016). Pengaruh Konsentrasi Sukrosa dan Sirup Glukosa Terhadap Sifat Kimia dan Sensoris Permen Keras Belimbing Wuluh (Averrhoa bilimbi. L). Teknologi Pertanian Fakultas Pertanian Universitas Sam Ratulangi, 1–10.
Fitriani, A., Supriyanti, F.M.T., & Heryanto, T.E. (2013). Penentuan Aktivitas Amilase Kasar Termofil Bacillus subtilis Isolat Kawah Gunung Darajat Garut, Jawa Barat. Bionatura-Jurnal Ilmu-Ilmu Hayati dan Fisik, 15(2), 107–113.
Hemalatha, M., & Visantini, P. (2020). Potential Use of Eco-enzyme for the Treatment of Metal Based Effluent. IOP Conf. Series: Materials Science and Engineering, 716, 1-6. https://doi:10.1088/1757-899X/716/1/012016
Istia’nah, D., Utami, U., & Barizi, A. (2020). Karakterisasi Enzim Amilase dari Bakteri Bacillus megaterium pada Variasi Suhu, pH dan Konsentrasi Substrat. Jurnal Riset Biologi Dan Aplikasinya, 2(1), 11. https://doi.org/10.26740/jrba.v2n1.p11-17
Jadid, N., Jannah, A. L., Handiar, B. P. W. P., Nurhidayati, T., Purwani, K. I., Ermavitalin, D., & Navastara, A. M. (2022). Aplikasi Eco Enzyme sebagai Bahan Pembuatan Sabun Antiseptik. Sewagati, 6(1), 69-75.
Kusumaningrum, A., Gunam, I. B. W., & Wijaya, I. M. M. (2019). Optimization of Temperature and pH on Endoglucanase Enzyme Activity Using Response Surface Methodology (RSM). Jurnal Rekayasa dan Manajemen Agroindustri, 7(2), 243-253.
Larasati, D., Astuti, A. P., & Maharani, E. T. (2020). Uji Organoleptik Eco-Enzyme dari Limbah Kulit Buah (Studi Kasus di Kota Semarang). Seminar Nasional Edusainstek, 278–283. https://doi.org/10.31539/bioedusains.v5i1.3387
Layly, I. R., & Wiguna, N. O. (2016). Studi Potensi Lipase Alcaligenes faecalis Untuk Aplikasi Biodeterjen. Jurnal Bioteknologi & Biosains Indonesia, 3(2), 66–71.
Lestari, P., Raharjo, T. K., Matsjeh, S. and Haryadi. W. (2016). Partial Purification and Biochemical Characterization of Extracellular Lipase from Azospirillum sp. JG3 Bacteria. Advances of Science and Technology for Society. AIP Conf. Proc.
Nangin, D., & Sutrisno, A. (2015). Enzim Amilase Pemecah Pati Mentah dari Mikroba : Kajian Pustaka. Jurnal Pangan Dan Agroindustri, 3(3), 1032–1039.
Nazim, F., & Meera, V. (2015). Use of Garbage Enzyme as a Low Cost Alternative Method for Treatment of Greywater - A review. Journal of Environmental Science and Engineering.
Nurdin, Nasihin, L., Herlina, N., Supartono, T., Kosasih, D., & Nurlaila, A. (2021). Pemanfaatan Sampah Organik Sebagai Biohandsanitizer dan Biodesinfektan Berbasis Eco-Community Untuk Mencegah Penyebaran Virus Corona. Jurnal Berdaya Mandiri, 3(2), 578–587.
Nurhamidah, Amida, N., Rohiat, S., & Elvinawati. (2021). Pengolahan Sampah Organik Menjadi Eco-Enzyme pada Level Rumah Tangga menuju Konsep Eco-Community. Andromeda, 1(2), 43–46.
Nurkhotimah, Yulianti, E., & Rakhmawati, A. (2017). The Influence of Temperature and pH Toward the Activity of the Phosphatase Enzyme from Thermophilic Bacteria of Gendol River Pasca Merapi Eruption. Jurnal Prodi Biologi, 6(8),465-471.
Pagarra, H., Hartati & Rachmawaty. (2021). Optimization of Protease Enzymes by Endophytic Bacteria from Roots of Karst Ecosystem Areas with Response Surface Methodology. Jurnal Sainsmat, 10(2),140-151.
Penitobe, T. (2021). Pengaruh Penambahan Enzim Protease Kasar (Crude Protease Enzyme) dari Daun Kelor (Moringga olivera) Terhadap Uji Organoleptik dan Kadar Asam Laurat Pada VCO (Virgin Coconut Oil). Skripsi, 3(April), 49–58.
Pratamadina, E., & Wikaningrum, T. (2022). Potensi Penggunaan Eco Enzyme pada Degradasi Deterjen dalam Air Limbah Domestik. Jurnal Serambi Engineering, 7(1), 2722–2728. https://doi.org/10.32672/jse.v7i1.3881
Purwanto, M. G. M. (2014). Perbandingan Analisa Kadar Protein Terlarut Dengan Berbagai Spektroskopi UV-Visible. Jurnal Ilmiah Sains dan Teknologi, 7(2), 64-71.
Rasit, N., Fern, L. H., & Ghani, W. A. W. A. K. (2019).
Rochyani, N., Utpalasari, R. L., & Dahliana, I. (2020). Analisis Hasil Konversi Eco Enzyme Menggunakan Nenas (Ananas comosus) dan Pepaya (Carica papaya L.). Jurnal Redoks, 5(2), 135–140.
Sari, E. M., & Fadhilah, T. M. (2021). Penentuan Kadar Protein Albumin dalam Sampel Brownies yang Diberikan Kepada Penderita Tuberkulosis. Chimica et Natura Acta, 9(2), 45-49. https://doi.org/10.24198/cna.v9.n2.34096
Soeka, Y. S., & Sulistiani, S. (2017). Karakterisasi Enzim Protease dari Bakteri Stenotrophomonas sp. Asal Gunung Bromo, Jawa Timur. LIPI, 16(2). https://doi.org/10.14203/beritabiologi.v16i2.2940
Soleha, S., & Retnaningrum, E. (2020). Optimization Extracellular Lipase Activity from Moraxella sp SBE01 for Hydrocarbons Nanoremediation. AIP Conference Proceedings 2260, 1 – 7. https://doi.org/10.1063/5.0015926.
Sumardi, Farisi S., Ekowati, C. N., & Diana, M. S. (2019). Aktivitas dan Karakterisasi Enzim Protease Isolat Bacillus sp. (UJ132) Secara Kualitatif dan Kuantitatif. Jurnal Riset Akuakultur, 14 (3), 193-199. http://ejournal-balitbang.kkp.go.id/index.php/jra
Sumarlin, L. O. (2008). Aktivitas Protease dari Bacillus circulans pada Media Pertumbuhan Dengan pH Tidak Terkontrol. Jurnal Kimia VALENSI, 1(2), 58–62. https://doi.org/10.15408/jkv.v1i2.215
Supriyatna, A., Amalia, D., Jauhari, A. A., & Holydaziah, D. (2015). Aktivitas Enzim Amilase, Lipase, dan Protease dari Larva Hermetia illucens yang Diberi Pakan Jerami Padi. Edisi Juli 2015, IX(2), 18–32.
Tazkiah, N. P., Rosahdi, T. D., & Supriadin, A. (2017). Isolasi dan Karakterisasi Enzim Amilase dari Biji Nangka (Artocarpus heterophillus). Al-Kimiya, 4(1), 17–22. https://doi.org/10.15575/ak.v4i1.5079
Tondais, J., Sombo, D. E., Lalenoh, B. A., Mappiratu, M., Adrian, A., & Cahyono, E. (2020). Ekstraksi Flavour dari Tepung Ikan Layang (Decapterus sp.) Menggunakan Enzim Protease Biduri (Calotropis gigantea). Jurnal FishtecH, 9(1), 6–12. https://doi.org/10.36706/fishtech.v9i1.11481
Wahjuni, S., Suarya, P., & Saputra, I. M. A. (2017). Isolasi Enzim Amilase dari Kecambah Biji Jagung Lokal Seraya (Zea mays L.) untuk Hidrolisis Pati. Jurnal Kimia, 11(2), 122–128. https://www.ptonline.com/articles/how-to-get-better-mfi-results
Zainudin & Kesumaningwati, R. (2022). Effect of Eco Enzyme on Heavy Metal Content in Ex-Coal Mining Land. Ziraa’ah, 47 (2), 154-161